Interferon assay based on the inhibition of double-stranded reovirus RNA accumulation in mouse L cells.
Interferon assay based on the inhibition of double-stranded reovirus RNA accumulation in mouse L cells.
复制标题
基于抑制小鼠 L 细胞中双链呼肠孤病毒 RNA 积累的干扰素测定。
DOI：
10.1099/0022-1317-19-1-81
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
P. Lengyel
中科院分区：
文献类型：
--
作者：
A. Vassef;G. Beaud;K. Paucker;P. Lengyel
总结
Summary
Treatment of mouse L 929 cells in cell culture with mouse interferon, prior to infection with reovirus type 3, decreased the amount of virus progeny formed. In infected, interferon-treated cells less reovirus specified double-stranded RNA (ds reoRNA) and single-stranded RNA accumulated than in infected control cells and the rate of ds reoRNA accumulation was inversely related to the concentration of interferon with which the cells were treated prior to infection. Since ds reoRNA can be conveniently assayed, this forms the basis of a fast and simple assay for interferons.