Dynamics of membrane adhesion: the role of polyethylene glycol spacers, ligand-receptor bond strength, and rupture pathway.
Dynamics of membrane adhesion: the role of polyethylene glycol spacers, ligand-receptor bond strength, and rupture pathway.
复制标题
膜粘附动力学：聚乙二醇间隔基的作用、配体-受体键强度和破裂途径。
DOI：
10.1021/la702357a
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Kuhl,TonyaL
中科院分区：
文献类型：
--
作者：
Wong,JoyceY;Kuhl,TonyaL
生物粘附通常通过相邻膜上的互补分子之间的离散交叉桥发生。在这里，我们报告使用表面力装置技术对柔性聚合物系链上的配体功能化的脂质膜与带有互补受体的第二膜之间的结合距离进行定量测量。显示结合距离作为聚合物系链长度的函数而增加。分离时，粘合失效不是在强配体-受体键处发生，而是主要通过从膜上机械拉出跨桥聚合物系链而发生。我们使用能量状态图总结了互补膜粘附动力学的测量结果，该能量状态图包含聚合物系链的能量、配体-受体键强度和形成的跨桥数量。
Biological adhesion typically occurs through discrete cross bridges between complementary molecules on adjacent membranes. Here we report quantitative measurements of the binding distance between a lipid membrane functionalized with ligands on flexible polymer tether chains and a second membrane bearing complementary receptors using the surface force apparatus technique. The binding distance is shown to increase as a function of polymer tether length. Upon separation, adhesive failure occurs not at the strong ligand−receptor bond but primarily through the mechanical pullout of cross-bridging polymer tethers from the membrane. We summarize these measurements of complementary membrane adhesion dynamics using an energy-state diagram that encompasses the energetics of the polymer tether, ligand−receptor bond strength, and number of cross bridges formed.