Variovorax guangxiensis sp nov., an aerobic, 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from banana rhizosphere
Variovorax guangxiensis sp nov., an aerobic, 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from banana rhizosphere
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DOI：
10.1007/s10482-014-0304-3
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发表时间：
2015-01-01
影响因子：
2.6
通讯作者：
Sun, Jian-guang
中科院分区：
文献类型：
--
作者：
Gao, Jun-lian;Yuan, Mei;Sun, Jian-guang
从广西香蕉根际分离到一株1-氨基环丙烷-1-羧酸脱氨酶产生菌，命名为GXGD 002（T）。基于16 S rRNA基因序列的系统发育分析表明，菌株GXGD 002（T）属于Variovorax属。GXGD 002（T）与Variovorax paradoxus DSM 30034（T）的16 S rRNA基因序列高度相似（99.4%），人参果腐病菌KCTC 12583（T）（99.1%），硼丛枝菌KCTC 22010（T）（99.0%）、土壤食虫菌DSM 18216（T）（98.7%）、德氏食虫菌DSM 27259（T）（98.1%）和多克顿食虫菌KCTC 12544（T）（97.4%）。然而，菌株GXGD 002（T）与其近缘种奇异弧菌DSM 30034（T）、人参弧菌KCTC 12583（T）和硼积累弧菌KCTC 22010（T）之间的DNA-DNA杂交值分别为40.7%、30.9%和23.7%。菌株GXGD 002（T）的DNA G + C含量为67.8mol%。菌株GXGD 002（T）的主要脂肪酸为C-16：0（20.3%）、C-10：0 3OH（18.4%）、C-17：0环状（18.9%）、C（18：1）w（7）c（12.3%）和总特征3（13.9%）。主要的呼吸醌被鉴定为泛醌-8（Q-8），主要的极性脂质为磷脂酰乙醇胺和磷脂酰甘油。通过生理生化、全细胞SDS-PAGE电泳和化学分类学等多相分类学研究，将GXGD 002（T）菌株与Variovorax属的其它种进行了比较。在此基础上提出了一个新种--广西多食蚜蝇Variovorax guangxiensis。模式菌株为GXGD 002（T）（=DSM 27352（T）= ACCC 05911（T））。
A 1-aminocyclopropane-1-carboxylate deaminase producing bacterium, designated GXGD002(T), was isolated from the rhizosphere of banana plants cultivated in Guangxi province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXGD002(T) is a member of the genus Variovorax. High levels of 16S rRNA gene sequence similarity are found between strain GXGD002(T) and Variovorax paradoxus DSM 30034(T) (99.4 %), Variovorax ginsengisoli KCTC 12583(T) (99.1 %), Variovorax boronicumulans KCTC 22010(T) (99.0 %), Variovorax soli DSM18216(T) (98.7 %), Variovorax defluvii DSM 27259(T) (98.1 %) and Variovorax dokdonensis KCTC 12544(T) (97.4 %) respectively. However, the DNA-DNA hybridization values between strain GXGD002(T) and its closely related species V. paradoxus DSM 30034(T), V. ginsengisoli KCTC 12583(T) and V. boronicumulans KCTC 22010(T) were found to be 40.7, 30.9 and 23.7 %, respectively. The DNA G + C content of strain GXGD002(T) was found to be 67.8 mol%. The major fatty acids of strain GXGD002(T) are C-16:0 (20.3 %), C-10:0 3OH (18.4 %), C-17:0 cyclo (18.9 %), C(18:1)w(7)c (12.3 %) and summed feature 3 (13.9 %). The predominant respiratory quinone was identified as ubiquinone-8 (Q-8) and the major polar lipids as phosphatidylethanolamine and phosphatidylglycerol. The results of polyphasic taxonomic study including physiological and biochemical tests, whole-cell SDS-PAGE profiles and chemotaxonomic analysis allowed a clear differentiation of strain GXGD002(T) from the other species in the genus Variovorax. Based on these results, a new species, Variovorax guangxiensis, is proposed. The type strain is GXGD002(T) (=DSM 27352(T) = ACCC 05911(T)).