Homologue of mammalian apolipoprotein A-II in non-mammalian vertebrates
Homologue of mammalian apolipoprotein A-II in non-mammalian vertebrates
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DOI：
10.1093/abbs/gmp015
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发表时间：
2009-05-01
影响因子：
3.7
通讯作者：
Ando, Seiichi
中科院分区：
文献类型：
--
作者：
Choudhury, Malay;Yamada, Shoji;Ando, Seiichi
虽然载脂蛋白与分子量14 kDa（载脂蛋白-14 kDa）与鱼类血浆高密度脂蛋白（HDL），它仍然有待确定是否载脂蛋白-14 kDa的哺乳动物apoA-II的同源物。我们获得了日本鳗鲡和虹鳟apo-14 kDa的cDNA全序列。在DDBJ/EMBL/GenBank或TGI数据库中的14种鱼类中发现了日本鳗鲡apo-14 kDa序列的同源物。鱼载脂蛋白-14 kDa缺乏前肽，含有更多的内部重复比哺乳动物apoA-II。然而，系统发育分析允许鱼载脂蛋白-14 kDa的同源物的哺乳动物载脂蛋白A-II。此外，TGI、Ensembl或NCBI数据库的计算机克隆揭示了狗、鸡、绿色变色蜥蜴和非洲爪蛙中的apoA-II，其序列迄今为止还不可用，这表明apoA-I和apoA-II都是脊椎动物HDL的基本成分。
Although apolipoprotein with molecular weight 14 kDa (apo-14 kDa) is associated with fish plasma high-density lipoproteins (HDLs), it remains to be determined whether apo-14 kDa is the homologue of mammalian apoA-II. We have obtained the full cDNA sequences that encode Japanese eel and rainbow trout apo-14 kDa. Homologues of Japanese eel apo-14 kDa sequence could be found in 14 fish species deposited in the DDBJ/EMBL/GenBank or TGI database. Fish apo-14 kDa lacks propeptide and contains more internal repeats than mammalian apoA-II. Nevertheless, phylogenetic analysis allowed fish apo-14 kDa to be the homologue of mammalian apoA-II. In addition, in silico cloning of the TGI, Ensembl, or NCBI database revealed apoA-IIs in dog, chicken, green anole lizard, and African clawed frog whose sequences had not so far been available, suggesting both apoA-I and apoA-II as fundamental constituents of vertebrate HDLs.