"Light-on" Sensing of Antioxidants Using Gold Nanoclusters
"Light-on" Sensing of Antioxidants Using Gold Nanoclusters
复制标题
DOI：
10.1021/ac500528m
复制
发表时间：
2014-05-20
影响因子：
7.4
通讯作者：
Khashab, Niveen M.
中科院分区：
文献类型：
--
作者：
Hu, Lianzhe;Deng, Lin;Khashab, Niveen M.
细胞内抗氧化剂的消耗与主要的细胞毒性事件和细胞紊乱有关，例如氧化应激和多发性硬化。除了医学诊断，确定食品、食品防腐剂和化妆品中抗氧化剂的浓度已被证明是非常重要的。金纳米团簇（Au-NC）具有小于2nm的核心尺寸，并且含有几个金属原子。它们具有有趣的生物物理性质，易于功能化，并且在各种生物医学应用中使用是安全的。在此，开发了一种基于Au-NCs的简单定量光谱方法来检测和成像抗氧化剂，如抗坏血酸。传感机制是基于抗氧化剂可以保护Au-NCs的荧光免受高活性氧猝灭的事实。我们的方法显示出很大的准确性时，用于检测商业果汁中的总抗氧化能力。此外，共聚焦荧光显微镜图像的HeLa细胞表明，这种方法可以成功地用于图像的抗氧化剂水平在活细胞。最后，以金纳米粒子的荧光强度为输出，讨论了这种“光开启”检测方法在多逻辑门制造中的潜在应用。
Depletion of intracellular antioxidants is linked to major cytotoxic events and cellular disorders, such as oxidative stress and multiple sclerosis. In addition to medical diagnosis, determining the concentration of antioxidants in foodstuffs, food preservatives, and cosmetics has proved to be very vital. Gold nanoclusters (Au-NCs) have a core size below 2 nm and contain several metal atoms. They have interesting photophysical properties, are readily functionalized, and are safe to use in various biomedical applications. Herein, a simple and quantitative spectroscopic method based on Au-NCs is developed to detect and image antioxidants such as ascorbic acid. The sensing mechanism is based on the fact that antioxidants can protect the fluorescence of Au-NCs against quenching by highly reactive oxygen species. Our method shows great accuracy when employed to detect the total antioxidant capacity in commercial fruit juice. Moreover, confocal fluorescence microscopy images of He La cells show that this approach can be successfully used to image antioxidant levels in living cells. Finally, the potential application of this "light-on" detection method in multiple logic gate fabrication was discussed using the fluorescence intensity of Au-NCs as output.