Importance of murine Vδ1+ γδ T cells expressing interferon-γ and interleukin-17A in innate protection against Listeria monocytogenes infection
Importance of murine Vδ1+ γδ T cells expressing interferon-γ and interleukin-17A in innate protection against Listeria monocytogenes infection
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DOI：
10.1111/j.1365-2567.2008.02841.x
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发表时间：
2008-10-01
期刊：
影响因子：
6.4
通讯作者：
Matsuzaki, Goro
中科院分区：
文献类型：
--
作者：
Hamada, Satoru;Umemura, Masayuki;Matsuzaki, Goro
小鼠伽玛-德尔塔T细胞参与针对细胞内单核细胞增多性李斯特菌感染的先天免疫反应。共表达V-γ-6的V-γ-1(+)-γ-T细胞是单核细胞增多性李斯特菌感染小鼠肝脏早期诱导的一个主要的-γ-T细胞亚群。为了探讨V-Gamma 6/V Delta 1(+)Gamma Delta T细胞对单核细胞增多性李斯特氏菌感染的保护作用，对V Delta 1基因缺陷(V Delta 1(-/-))小鼠进行了分析，因为这些小鼠在感染单核细胞增多性李斯特菌的肝脏中选择性地缺乏V Gamma 6/V Delta 1(+)Gamma Delta T细胞亚群。与野生型小鼠相比，V delta1(-/-)小鼠肝脏和脾中的细菌负荷增加，单核细胞增多性李斯特菌感染早期存活率下降。组织学检查发现V delta1(-/-)小鼠肝脏内可见脓肿样病变，巨噬细胞散在分布，而野生型小鼠未见。V-Gamma 6/V-Delta 1(+)-Gamma-Delta T细胞产生干扰素-γ和白介素17A。以上结果提示，小鼠V-γ6/V-Delta 1(+)-γ-Delta T细胞通过在感染肝脏产生促炎细胞因子干扰素-γ和白介素17A来控制对单核细胞增多性李斯特菌感染的先天保护反应。
Murine gamma delta T cells participate in the innate immune response against infection by an intracellular pathogen Listeria monocytogenes. V delta 1(+)gamma delta T cells coexpressing V gamma 6 are a major gamma delta T-cell subpopulation induced at an early stage of L. monocytogenes infection in the livers of infected mice. To investigate the protective role of the V gamma 6/V delta 1(+)gamma delta T cells against L. monocytogenes infection, V delta 1 gene-deficient (V delta 1(-/-)) mice were analysed because these mice selectively lacked a V gamma 6/V delta 1(+)gamma delta T-cell subpopulation in the L. monocytogenes-infected liver. The V delta 1(-/-) mice showed increased bacterial burden in the liver and spleen, and decreased survival rate at an early stage of L. monocytogenes infection when compared to wild-type mice. Histological examination showed abscess-like lesions and unorganized distribution of macrophages in the liver of the V delta 1(-/-) mice but not in the wild-type mice after L. monocytogenes infection. The V gamma 6/V delta 1(+)gamma delta T cells produced interferon-gamma and interleukin-17A. All the results suggest that murine V gamma 6/V delta 1(+)gamma delta T cells control the innate protective response against L. monocytogenes infection through production of the proinflammatory cytokines interferon-gamma and interleukin-17A in the infected liver.