Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte
Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte
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DOI：
10.1242/dev.01820
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发表时间：
2005-05-01
期刊：
影响因子：
4.6
通讯作者：
Nilson, LA
中科院分区：
文献类型：
--
作者：
Cáceres, L;Nilson, LA
果蝇卵母细胞发育中的 gurken mRNA 和蛋白质的不对称定位决定了未来胚胎的前后轴和背腹轴。了解这些不对称的根源需要了解古尔肯转录本的来源。在卵子发生过程中，卵母细胞中的大多数转录物是由相关的护士细胞产生的，但有人提出，gurken 是一种特殊的卵母细胞衍生的转录物。使用标准有丝分裂重组技术的新应用，我们生成了嵌合卵室，其中护士细胞（而不是卵母细胞）可以产生古尔肯。 Gurken 正确定位在这些嵌合体中，并且卵母细胞轴向极性正常建立，表明护士细胞合成 Gurken，并且它们的贡献足以实现 Gurken 功能。我们的数据证明存在一种从护士细胞转运格肯及其随后在卵母细胞内定位的机制。
The asymmetric localization of gurken mRNA and protein in the developing Drosophila oocyte defines both the anteroposterior and dorsoventral axes of the future embryo. Understanding the origin of these asymmetries requires knowledge of the source of gurken transcripts. During oogenesis most transcripts in the oocyte are produced by the associated nurse cells, but it has been proposed that gurken is an exceptional oocyte-derived transcript. Using a novel application of a standard mitotic recombination technique, we generated mosaic egg chambers in which the nurse cells, but not the oocyte, could produce gurken. Gurken was properly localized in these mosaics and oocyte axial polarity was established normally, indicating that the nurse cells synthesize gurken and that their contribution is sufficient for Gurken function. Our data demonstrate the existence of a mechanism for transport of gurken from the nurse cells and its subsequent localization within the oocyte.