Morphology of dry solid-supported protein monolayers dependent on the substrate and protein surface properties.
Morphology of dry solid-supported protein monolayers dependent on the substrate and protein surface properties.
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干固相支持的蛋白质单层的形态取决于基质和蛋白质表面特性
DOI：
10.1021/la0530182
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Morbitzer
中科院分区：
文献类型：
--
作者：
Schönafinger;Morbitzer
用原子力显微镜研究了不同固体基质上干燥的MERGA蛋白质单分子膜的形态。MRGA是一种十二聚体储铁蛋白，在低过饱和度时可在亲水表面形成六方二维晶单分子膜。这种二维晶体的形成在很大程度上取决于孵化液的pH值和盐度以及表面性质。表面覆盖度与底物电荷、离子强度和pH的相关性表明，在吸附过程中，静电效应占主导地位，分子间排斥力和蛋白质−底物吸引力之间的平衡发生转移。在MRGA等电点附近，有利于吸附到表面并形成2D晶体。通过在模板剥离的金表面制备具有不同端基的硫醇自组装单分子膜，表面性质可以很容易地从高亲和力到极低的蛋白质亲和力变化。由此得到的结晶蛋白质结构的图案是新颖的，可以作为进一步科学研究的起点，例如固体支撑的与DNA的共晶，甚至是技术应用的发展，例如介观结构沉积MR-Ga笼形纳米颗粒。
The morphologies of dry MrgA protein monolayers on different solid substrates prepared by a three-step procedure (adsorption from an incubation solution, rinsing to remove excess salt and protein, and drying) were investigated using atomic force microscopy. MrgA is a dodecameric iron-storage protein which can form hexagonal, two-dimensional (2D) crystalline monolayers on hydrophilic surfaces at low supersaturation. The formation of such two-dimensional crystals is heavily dependent on the pH and the salinity of the incubation solution as well as on the surface properties. Correlation of surface coverage with substrate charge, ionic strength, and pH indicates the dominance of electrostatic effects in adsorption, with the balance shifting between intermolecular repulsion and protein−substrate attraction. Close to the isoelectric point (pI) of MrgA, adsorption to the surface and the formation of 2D crystals are favored. By preparation of self-assembled monolayers of thiols with different end groups on template-stripped gold, the surface properties can be varied easily from high to very low protein affinity. The resulting patterns of the crystalline protein structures are novel and could be a starting point for further scientific study, e.g., solid-supported cocrystallization with DNA, and indeed developments with technological applications, such as mesostructured deposition of MrgA-caged nanoparticles.