Preparation, characterization, and in vivo evaluation of nano formulations of ferulic acid in diabetic wound healing
Preparation, characterization, and in vivo evaluation of nano formulations of ferulic acid in diabetic wound healing
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DOI：
10.1080/03639045.2018.1496448
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发表时间：
2018-01-01
影响因子：
3.4
通讯作者：
Mishra, Brahmeshwar
中科院分区：
文献类型：
--
作者：
Bairagi, Ujjawal;Mittal, Pooja;Mishra, Brahmeshwar
糖尿病是最常见的以高血糖为特征的疾病。慢性高血糖可导致自由基的过度产生，从而导致氧化应激，从而损害伤口愈合。阿魏酸（FA）已被证明具有抗糖尿病和抗氧化特性。本研究的目的是制备阿魏酸纳米粒，并研究其降血糖和伤口愈合活性。采用纳米沉淀法制备阿魏酸-聚乳酸-羟基乙酸共聚物（FA-PLGA）纳米粒。制备的FA-PLGA纳米颗粒的平均粒径为240 nm。扫描电子显微镜（SEM）和原子力显微镜（AFM）分析表明所制备的FA-PLGA纳米粒为球形。药物包封实验表明，88.49%的FA被包裹在PLGA中。Carbopol 980用于配制FA-PLGA纳米颗粒负载的水凝胶。发现与糖尿病伤口对照组相比，经FA负载的聚合物纳米颗粒分散体（口服施用）和基于FA负载的聚合物纳米颗粒的水凝胶（局部施用）处理的伤口更快地上皮化。与糖尿病伤口对照组相比，羟脯氨酸含量显著增加。因此，结果表明FA显著促进糖尿病大鼠伤口愈合。
Diabetes mellitus is most common disorder characterize by hyperglycemia. Chronic hyperglycemia may lead to over production of free radicals thereby results in oxidative stress which impaired healing of wounds. Ferulic acid (FA) has been shown to have antidiabetic and antioxidant properties. The aim of the present study was to develop Ferulic acid nanoparticles and to study its hypoglycemic and wound healing activities. Ferulic acid-poly(lactic-co-glycolic acid) (FA-PLGA) nanoparticles were prepared by nano precipitation method. The prepared FA-PLGA nanoparticles had an average size of 240 nm. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) analysis showed the prepared FA-PLGA nanoparticles were spherical in shape. Drug encapsulation assay showed that 88.49% FA was encapsulated in PLGA. Carbopol 980 was used to formulate FA-PLGA nanoparticles loaded hydrogel. FA-loaded polymeric nanoparticles dispersion (oral administration) and FA-loaded polymeric nanoparticles based hydrogel (topical administration) treated wounds were found to epithelize faster as compared with diabetic wound control group. The hydroxyproline content increased significantly when compared with diabetic wound control. Therefore, the results indicate that FA significantly promotes wound healing in diabetic rats.