Expression of retinaldehyde dehydrogenase 1 in the anterior pituitary glands of adult rats
Expression of retinaldehyde dehydrogenase 1 in the anterior pituitary glands of adult rats
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DOI：
10.1007/s00441-007-0423-5
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发表时间：
2007-08-01
影响因子：
3.6
通讯作者：
Yashiro, Takashi
中科院分区：
文献类型：
--
作者：
Fujiwara, Ken;Kikuchi, Motoshi;Yashiro, Takashi
视黄酸（Retinoic acid， RA）在细胞生长和组织发育中起着至关重要的作用，也是垂体功能的调节因子。然而，RA是否在垂体中产生并作为旁分泌和/或自分泌因子发挥作用通常是未知的。RA是由类维生素a通过氧化过程合成的。催化视网膜氧化为RA的脱氢酶是视黄醛脱氢酶（RALDH）家族的成员。最近，我们证实RALDH2和RALDH3在大鼠发育中的垂体前叶中有表达，而RALDH1不表达，但RALDHs在成年垂体中的表达尚未确定。因此，我们现在检测了成年大鼠脑垂体中RALDH1、RALDH2和RALDH3 mrna的表达。通过实时定量聚合酶链反应分析成人垂体显示高水平的RALDH1 mRNA，而不高水平的RALDH2 mRNA和RALDH3 mRNA。我们还通过地高辛标记的cRNA探针原位杂交检测了垂体前叶中RALDH1的mRNA表达。对RALDH1 mRNA和垂体激素或S-100蛋白（卵泡星状细胞的标记物）进行双染色，发现RALDH1 mRNA在部分催乳素产生细胞、边缘层细胞和卵泡星状细胞中表达。我们的研究结果表明，RA产生于成人垂体前腺，并可能局部作用于垂体细胞。
Retinoic acid (RA) plays a critical role in cell growth and tissue development and is also a regulatory factor of pituitary function. However, whether RA is generated in the pituitary gland and plays a role as a paracrine and/or autocrine factor is generally unknown. RA is synthesized from retinoids through oxidation processes. Dehydrogenases that catalyze the oxidation of retinal to RA are members of the retinaldehyde dehydrogenase (RALDH) family. Recently, we demonstrated that RALDH2 and RALDH3, but not RALDH1, were expressed in the developing anterior pituitary gland of rats, but the expression of RALDHs in the adult pituitary gland was not determined. Therefore, we have now examined the expression of RALDH1, RALDH2, and RALDH3 mRNAs in the pituitary gland of adult rats. Analysis by quantitative real-time polymerase chain reaction of adult pituitary glands has revealed a high level of RALDH1 mRNA but not of RALDH2 mRNA or RALDH3 mRNA. We have also detected mRNA expression for RALDH1 in the anterior pituitary gland by in situ hybridization with digoxigenin-labeled cRNA probes. Double-staining for RALDH1 mRNA and pituitary hormones or S-100 protein, a marker of folliculo-stellate cells (FS-cells), has revealed RALDH1 mRNA expression in a portion of prolactin-producing cells, marginal layer cells, and FS-cells. Our results suggest that RA is generated in the adult anterior pituitary gland, and that it may act locally on pituitary cells.