2-Bromopropane induced germ cell apoptosis during spermatogenesis in male rat
2-Bromopropane induced germ cell apoptosis during spermatogenesis in male rat
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DOI：
10.1292/jvms.63.373
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发表时间：
2001-04-01
影响因子：
1.2
通讯作者：
Lee, YS
中科院分区：
文献类型：
--
作者：
Li, GX;Kang, KS;Lee, YS
2-溴丙烷（2-BP）可导致人类和大鼠睾丸毒性。然而，2-BP对睾丸生殖细胞的毒性作用尚不清楚。以135、405和1，355 mg/kg/天的剂量每天皮下注射2-BP至Sprague-Dawley大鼠，持续28天。在1，355 mg/kg/天剂量下，2-BP显著降低了体重和睾丸、附睾、精囊和前列腺的重量以及每日精子产量。在暴露于405 mg/ kg/天和1，355 mg/kg/天2-BP的大鼠睾丸中观察到生精小管萎缩伴生殖细胞（如精原细胞、精母细胞和细长精子细胞）变性。TUNEL阳性生殖细胞出现在405和1，355 mg/kg/天2-BP给药组中。电镜观察凋亡生殖细胞的超微结构改变。暴露28天后，在1，355 mg/kg/天剂量下观察到珠状细长精子细胞。这些结果表明，2-BP损害精子发生可能是由于凋亡的生殖细胞死亡。
2-Bromopropane (2-BP) causes testicular toxicity in humans and rats. However, the germ cell degeneration of testicular toxicity by 2-BP has not been understood. 2-BP at doses of 135, 405, and 1,355 mg/kg/day was daily injected subcutaneously into Sprague-Dawley rats for 28 days. At the dose of 1,355 mg/kg/day, 2-BP significantly decreased the weights of body and testes, eipididymis, seminal vesicle, and prostate, as well as daily sperm production. Atrophy of seminiferous tubules accompanied with degeneration of germ cells such as spermatogonia, spermatocytes, and elongated spermatids was observed in the testes of rats exposed to the 405 mg/ kg/day and 1,355 mg/kg/day of 2-BP. TUNEL-positive germ cells were appeared in the 405 and 1,355 mg/kg/day of 2-BP-treated groups. In addition, ultrastructure alterations of apoptotic germ cells were observed by the electron microscopy study. Bead elongated spermatids were observed at 1,355 mg/kg/day after 28 days exposure. These results suggest that 2-BP impair spermatogenesis may result from apoptotic germ cell death.