Effect of volatile general anesthetics and n-alcohols on glutamate-stimulated increases in calcium ion flux in hippocampal membrane vesicles.
Effect of volatile general anesthetics and n-alcohols on glutamate-stimulated increases in calcium ion flux in hippocampal membrane vesicles.
复制标题
挥发性全身麻醉剂和正醇对谷氨酸刺激的海马膜囊泡中钙离子通量增加的影响。
DOI：
10.1159/000139277
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发表时间：
1995
期刊：
影响因子：
3.1
通讯作者：
Daniell,LC
中科院分区：
文献类型：
--
作者：
Daniell,LC
N-甲基D-天冬氨酸（NMDA）受体的活性测定L-谷氨酸刺激的增加，游离细胞内钙浓度（Cai）的膜囊泡分离自小鼠海马。与麻醉浓度的挥发性全身麻醉药，氟烷，恩氟烷，乙醚，抑制NMDA反应。正醇类、乙醇、丙醇、丁醇和戊醇抑制NMDA受体反应，而甲醇无效。挥发性麻醉剂和正-醇抑制NMDA反应的能力似乎与其中一些药物增加静息Caisin的能力无关，因为这两种作用之间没有相关性。本研究的结果表明，海马NMDA受体是敏感的挥发性全身麻醉药和n-醇抑制。这种作用可能部分地是它们在体内的麻醉作用的基础。
N-Methyl D-aspartate (NMDA) receptor activity was determined by measurement of L-glutamate-stimulated increases in free intracellular calcium concentrations (Cai) in membrane vesicles isolated from mouse hippocampi. Incubation with anesthetic concentrations of the volatile general anesthetics, halothane, enflurane, and diethyl ether, inhibited NMDA responses. The n-alcohols, ethanol, propanol, butanol and pentanol inhibited NMDA receptor responses, but methanol was ineffective. The ability of volatile anesthetics and n-alcohols to inhibit NMDA responses appears to be unrelated to the ability of some of these agents to increase resting Caisince no correlation occurred between the two effects. The results of this study show that hippocampal NMDA receptors are sensitive to inhibition by volatile general anesthetics and n-alcohols. This effect may, in part, underlie their anesthetic effects in vivo.