Self-sufficient, low-cost microfluidic pumps utilising reinforced balloons
Self-sufficient, low-cost microfluidic pumps utilising reinforced balloons
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DOI：
10.1039/c9lc00618d
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发表时间：
2019-09-07
期刊：
影响因子：
6.1
通讯作者：
Khoshmanesh, Khashayar
中科院分区：
文献类型：
--
作者：
Thurgood, Peter;Suarez, Sergio Aguilera;Khoshmanesh, Khashayar
在这里，我们介绍了一种提高乳胶气球自给式压力泵充气压力的简单方法。我们的方法包括用弹性纤维加固乳胶气球，以限制气球的膨胀并增加其充气压力。这使得我们能够将乳胶气球的操作充气压力从2.5 kpa增加到25 kpa。概念验证实验表明，增强气球适合于诱导侧向力和回流，用于流体动力捕获大型人体单核细胞。我们还演示了在手动挤压增强气球后，在重复循环中快速交换溶液的能力。我们还显示了增强球囊在不同剪应力水平下适合于研究人主动脉内皮细胞的力学生物学。增强型气球泵的简单性、便携性、价格实惠、超弹性和可扩展性使其适合于广泛的微流体应用。
Here, we introduce a simple method for increasing the inflation pressure of self-sufficient pressure pumps made of latex balloons. Our method involves reinforcing the latex balloon with elastane fibres to restrict the expansion of the balloon and increase its inflation pressure. This allowed us to increase the operational inflation pressure of a latex balloon from 2.5 to 25 kPa. Proof-of-concept experiments show the suitability of the reinforced balloon for inducing lateral forces and recirculating flows, which are employed for hydrodynamic capturing of large human monocytes. We also demonstrate the ability for the rapid exchange of solutions in repeated cycles upon manual squeezing of the reinforced balloons. We also show the suitability of the reinforced balloon for studying the mechanobiology of human aortic endothelial cells under various shear stress levels. The simplicity, portability, affordability, hyper-elasticity and scalability of the reinforced balloon pumps make them suitable for a wide range of microfluidic applications.