GLYCOSAMINOGLYCANS OF NORMAL AND ARTHRITIC CARTILAGE
GLYCOSAMINOGLYCANS OF NORMAL AND ARTHRITIC CARTILAGE
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DOI：
10.1172/jci106660
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发表时间：
1971-01-01
影响因子：
15.9
通讯作者：
LIPPIELL.L
中科院分区：
文献类型：
--
作者：
MANKIN, HJ;LIPPIELL.L
本文分析了13例正常人和15例骨关节炎患者髋关节软骨中糖胺聚糖的含量和分布。在用链霉蛋白酶和木瓜蛋白酶消化组织后，通过Svejcar和Robertson的技术在短纤维素柱上用CPC洗脱来分离GAG。通过多种方法鉴定洗脱液，包括测定摩尔比、透明质酸酶处理后的N-乙酰基-己糖胺测定和未水解和水解的GAG的薄层色谱法。从获得的数据可以证明，来自关节炎患者的软骨显示出4-硫酸软骨素浓度的显著增加和硫酸角质素浓度的显著减少，存在的GAG总量仅有轻微变化。计算的摩尔比显示的硫酸化的变化与软骨素4-硫酸盐出现在“过硫酸化”状态在arthritic软骨。数据导致推测有关的过程中骨关节炎，它的结论是，看到的变化更可能代表一个改变的模式的合成，而不是选择性降解。由于这些变化表明软骨更年轻，因此提出了一种理论，即软骨细胞通过调节成软骨细胞阶段来响应骨关节炎的慢性应激。
The cartilages from the hip joints of 13 normal and 15 osteoarthritic humans were analyzed for glycosaminoglycan content and distribution. The GAGs were separated by elution with CPC on a short cellulose column by the technique of Svejcar and Robertson after digestion of the tissue with pronase and papain. The eluates were identified by a variety of methods including determination of molar ratios, N-acetyl-hexosamine determinations after hyaluronidase treatment and thin-layer chromatography of unhydrolyzed and hydrolyzed GAGs.From the data obtained, it was demonstrated that cartilage from arthritic patients showed a significant increase in the concentration of chondroitin 4-sulfate and a significant decrease in keratan sulfate, with only slight changes in the total amount of GAG present. Calculations of the molar ratios showed variation in the sulfation with chondroitin 4-sulfate appearing in the “supersulfated” state in the arthritic cartilage.The data lead to speculation regarding the process of osteoarthritis, and it is concluded that the changes seen are more likely to represent an altered pattern of synthesis rather than selective degradation. Since the changes suggest a younger cartilage, a theory is advanced that the chondrocyte responds to the chronic stress of osteoarthritis by modulation to a chondroblastic phase.