Modifying effects of fermented brown rice on fecal microbiota in rats
Modifying effects of fermented brown rice on fecal microbiota in rats
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DOI：
10.1016/j.anaerobe.2007.07.001
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发表时间：
2007-10-01
期刊：
影响因子：
2.3
通讯作者：
Ohnishi, Yoshinari
中科院分区：
文献类型：
--
作者：
Kataoka, Keiko;Kibe, Ryoko;Ohnishi, Yoshinari
曲霉发酵糙米是一种富含纤维的食品。研究了FBRA饮食给药对大鼠粪便微生物群组成的影响。雄性Wistar大鼠饲喂基础饮食或含5%FBRA或10%FBRA的饮食，并通过培养和末端限制性片段长度多态性（T-RFLP）分析来分析粪便微生物群。饲喂10%FBRA饲料3周后，乳酸杆菌活细胞数显著高于基础饲料组和试验开始时（第0天）。与基础饮食的效果相比，饲喂10%FBRA 12周后，还观察到乳酸杆菌活细胞数的增加。T-RFLP分析表明，在10%FBRA喂养14周的大鼠粪便中的乳酸杆菌细胞的百分比增加。根据随机扩增多态性DNA（RAPD）图谱将分离自大鼠粪便中的乳酸杆菌分为6种类型，分别鉴定为罗伊氏乳杆菌（Lactobacillitsreuteri）、罗伊氏乳杆菌（Lactobacillitsreuteri）、罗伊氏乳杆菌（LactobacillitsL.）基于16 S rDNA部分序列的同源性，将乳酸杆菌和乳酸杆菌种进行比较。FBRA中含有乳酸菌，但其RAPD图谱和鉴定菌种与大鼠粪便中的乳酸菌不同。这些结果表明，膳食FBRA增加了已经驻留在大鼠肠道中的乳酸杆菌种类的数量。(c)2007爱思唯尔有限公司保留所有权利。
Brown rice fermented by Aspergillus oryzae (FBRA) is a fiber-rich food. Effects of dietary administration of FBRA on rat fecal microbiota composition were examined. Male Wistar rats were fed a basal diet or a 5% FBRA- or 10% FBRA-containing diet, and fecal microbiota was analyzed by Culture and terminal-restriction fragment length polymorphism (T-RFLP) analysis. The viable cell number of lactobacilli significantly increased after feeding, 10% FBRA diet for 3 weeks compared with that in the basal diet group and that in the same group at the beginning of the experiment (day 0). An increase in the viable cell number of lactobacilli was also observed after feeding 10% FBRA for 12 weeks compared with the effect of a basal diet. T-RFLP analysis showed an increase in the percentage of lactobacilli cells in feces of rats fed 10% FBRA for 14 weeks. Lactobacilli strains isolated from rat feces were divided into six types based on their randomly amplified polymorphic DNA (RAPD) patterns, and they were identified as Lactobacillits reuteri, L. intestinalis and lactobacilli species based on homology of the partial sequence of 16S rDNA. FBRA contains lactic acid bacteria, but their RAPD patterns and identified species were different from those in rat feces. These results indicated that dietary FBRA increases the number of lactobacilli species already resident in the rat intestine. (c) 2007 Elsevier Ltd. All rights reserved.