Cell-surface proteins of Streptococcus sanguis associated with cell hydrophobicity and coaggregation properties.
Cell-surface proteins of Streptococcus sanguis associated with cell hydrophobicity and coaggregation properties.
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血链球菌的细胞表面蛋白与细胞疏水性和共聚集特性相关。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
H. Jenkinson
中科院分区：
文献类型：
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作者：
H. Jenkinson
在不引起裂解的条件下，用月桂酰肌氨酸钠孵育血链球菌细胞，溶解的物质占细胞干重的5-8%。处理降低了细胞疏水性，并降低了细胞与放线菌共聚集的能力。提取物中含有约20种多肽，根据以下一个或多个标准，这些多肽被鉴定为细胞表面成分：当细胞与蛋白酶孵育时被降解;当细胞使用乳过氧化物酶催化反应碘化时被标记;与固定全细胞的抗体反应。其中8种多肽占提取总蛋白的70%以上，1种组分（分子量16 kDa）为疏水性。所描述的细胞表面蛋白质与细胞疏水性和共聚集有关。
Incubating cells of Streptococcus sanguis with sodium lauroyl sarcosinate, under conditions that did not cause lysis, solubilized material comprising 5-8% of the cell dry weight. The treatment reduced cell hydrophobicity, and reduced the ability of the cells to coaggregate with Actinomyces spp. The extract contained about 20 polypeptides and these were identified as being cell-surface components on the basis of one or more of the following criteria: being degraded when cells were incubated with protease; being labelled when cells were iodinated using a lactoperoxidase-catalysed reaction; reacting with antibodies raised to fixed whole cells. Eight of the polypeptides accounted for more than 70% of the total protein extracted, and one component (molecular mass 16 kDa) was hydrophobic. The cell-surface proteins described are implicated in cell hydrophobicity and coaggregation.