GOOSECOID EXPRESSION IN NEURECTODERM AND MESENDODERM IS DISRUPTED IN ZEBRAFISH CYCLOPS GASTRULAS
GOOSECOID EXPRESSION IN NEURECTODERM AND MESENDODERM IS DISRUPTED IN ZEBRAFISH CYCLOPS GASTRULAS
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DOI：
10.1006/dbio.1994.1212
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发表时间：
1994-08-01
影响因子：
2.7
通讯作者：
POSTLETHWAIT, JH
中科院分区：
文献类型：
--
作者：
THISSE, C;THISSE, B;POSTLETHWAIT, JH
来自goosecoid的RNA，一种在脊椎动物胚胎前中胚层原肠胚形成期间表达的含有同源框的基因，当注入腹侧中胚层时可以产生组织者活性，导致次级体轴;然而，目前还不清楚goosecoid如何执行其组织者功能。我们在这里报告，在斑马鱼原肠胚，goosecoid表达域出现在假定的前神经外胚层直接位于goosecoid表达中内胚层细胞。从这个位置开始，类鹅的表达逐渐扩散到外胚层。在剑水蚤突变胚胎，缺乏腹前脑，goosecoid的表达是异常的中内胚层和完全不存在的覆盖neurectoderm。这些结果表明，剑水蚤是需要正确规范的中内胚层，并建议goosecoid表达的外胚层可能会导致从中胚层垂直诱导。我们建议，在原肠胚头，goosecoid可能是重要的组织腹神经外胚层。(C)1994年出版社出版。
RNA from goosecoid, a homeobox-containing gene expressed during gastrulation in the anterior mesoderm of vertebrate embryos, can generate organizer activity when injected into ventral mesoderm, resulting in a secondary body axis; it is not yet understood, however, how goosecoid performs its organizer function. We report here that in the zebrafish gastrula, a domain of goosecoid expression arises in presumptive anterior neurectoderm which lies directly above goosecoid-expressing mesendodermal cells. From this position, goosecoid expression then spreads gradually across the ectodermal layer. In cyclops mutant embryos, which lack a ventral anterior brain, expression of goosecoid is abnormal in the mesendoderm and completely absent in the overlying neurectoderm. These results indicate that cyclops is required for correct specification of the mesendoderm and suggest that goosecoid expression in the ectoderm may result from vertical induction from the mesoderm. We propose that in the gastrula head, goosecoid may be important in organizing the ventral neurectoderm. (C) 1994 Academic Press, Inc.