Osteoblastic activity and estrogenic response in the regenerating scale of goldfish, a good model of osteogenesis.
Osteoblastic activity and estrogenic response in the regenerating scale of goldfish, a good model of osteogenesis.
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DOI：
10.1016/j.lfs.2004.10.063
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发表时间：
2005-04
期刊：
影响因子：
6.1
通讯作者：
H. Yoshikubo;N. Suzuki;Keiju Takemura;M. Hoso;S. Yashima;S. Iwamuro;Y. Takagi;M. Tabata;A. Hattori
中科院分区：
文献类型：
--
作者：
H. Yoshikubo;N. Suzuki;Keiju Takemura;M. Hoso;S. Yashima;S. Iwamuro;Y. Takagi;M. Tabata;A. Hattori
用金鱼再生鳞片对硬骨鱼的成骨进行了形态学观察。组织学观察表明，第7 ~ 10天再生鳞片周围成骨细胞的大小和数量均大于其他阶段。因此，我们进一步研究成骨细胞在再生期的活性。为了量化成骨细胞的活性，取左侧体表，然后在第7、10和15天用再生体表测量成骨细胞的标志物碱性磷酸酶（ALP）的活性。在同一天，还收集了身体右侧的个体鳞片，并用于测量ALP活性。再生鳞片各阶段的成骨细胞均比其余成骨鳞片的成骨细胞活跃。第10天的再生尺度活性最高。此外，我们发现雌激素受体（ER） mRNA在再生鳞片中表达，因为雌激素参与哺乳动物成骨细胞的生长和分化。因此，使用先前报道的规模培养系统，在第10天的个体和再生规模上检查雌激素反应。在再生鳞片中的反应性要高得多，尽管雌激素治疗显著激活了这两个鳞片的成骨细胞活性。我们是第一个证明内质网在再生鳞片中表达，雌激素参与骨形成，就像在哺乳动物骨骼中一样。我们的研究结果强烈表明，再生鳞片可以用作脊椎动物成骨的模型。
Osteogenesis in the teleost was morphologically observed using regenerating scales of goldfish. Histological observations indicated that osteoblasts around the regenerating scales on days 7 to 10 were greater in size and number than those at other stages. Therefore, further experiments were carried out to examine the activity of osteoblasts in the regenerating period. To quantify their osteoblastic activities, scales on the left side of the body were taken, and the regenerating scales were then used to measure the activities of alkaline phosphatase (ALP), a marker of osteoblasts, on days 7, 10, and 15. The ontogenic scales on the right side of the body were also collected and used to measure ALP activity on the same days. Osteoblasts at all stages of regenerating scales were more active than those in the remaining ontogenic scales. The regenerating scales on day 10 had the highest activity. Furthermore, we found that estrogen receptor (ER) mRNA was expressed in the regenerating scales because estrogen participates in osteoblastic growth and differentiation in mammals. Therefore, using a scale culture system reported previously, the estrogenic response was examined in the ontogenic and regenerating scales on day 10. The reactivity was much higher in regenerating scales, although estrogen treatment significantly activated the osteoblastic activities in both scales. We are the first to demonstrate that ER is expressed in regenerating scales and that estrogen participates in osteogenesis as it does in mammalian bone. Our findings strongly suggest that regenerating scales can be used as a model of osteogenesis in vertebrates.