Evaluation of bioluminescence-based assays of anti-malarial drug activity.
Evaluation of bioluminescence-based assays of anti-malarial drug activity.
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评估抗疟疾药物活性的基于生物发光的测定法。
DOI：
10.1186/1475-2875-12-58
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发表时间：
2013-02-08
期刊：
影响因子：
3
通讯作者：
Horrocks P
中科院分区：
文献类型：
--
作者：
Hasenkamp S;Sidaway A;Devine O;Roye R;Horrocks P
Transgenic Plasmodium falciparum expressing luciferase offers an attractive bioluminescence-based assay platform for the investigation of the pharmacological properties of anti-malarial drugs. Here a side-by-side comparison of bioluminescence and fluorescence-based assays, utilizing a luciferase reporter cassette that confers a strong temporal pattern of luciferase expression during the S-phase of intraerythrocytic development, is reported. Key assay parameters for a range of commercially available luminogenic substrates are determined and compared to those measured using a Malaria Sybr Green I fluorescence assay. In addition, the short-term temporal effects of anti-malarial compounds are evaluated using both bioluminescent and fluorescent assay platforms. The Z’, % coefficient of variation and 50% inhibition concentrations are essentially the same for bioluminescent and fluorescent assays in transgenic parasites generated in both chloroquine-sensitive and -resistant genetic backgrounds. Bioluminescent assays, irrespective of the luminogenic agent employed, do, however, offer significantly enhanced signal-to-noise ratios. Moreover, the bioluminescent assay is more dynamic in terms of determining temporal effects immediately following drug perturbation. This study suggests that opportunities for bioluminescence-based assays lie not in the measurement of 50% inhibition concentrations, where the cheaper fluorescence assay performs excellently and is not restricted by the need to genetically modify the parasite clone under investigation. Instead, assays that use the dynamic response of the luciferase reporter for semi-automated screening of additional pharmacological properties, such as relative rate-of-kill and lethal dose estimation, are a more attractive development opportunity.
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影响因子：
5.6
作者：
Wong, Eleanor H.;Hasenkamp, Sandra;Horrocks, Paul
通讯作者：
Horrocks, Paul
影响因子：
3.7
作者：
Lelièvre J;Almela MJ;Lozano S;Miguel C;Franco V;Leroy D;Herreros E
通讯作者：
Herreros E
影响因子：
3
作者：
Hasenkamp S;Wong EH;Horrocks P
通讯作者：
Horrocks P
DOI：
10.1073/pnas.0802982105
发表时间：
2008-07-01
影响因子：
11.1
作者：
Plouffe, David;Brinker, Achim;Winzeler, Elizabeth A.
通讯作者：
Winzeler, Elizabeth A.
影响因子：
4.2
作者：
Burrows, Jeremy N.;Leroy, Didier;Waterson, David
通讯作者：
Waterson, David