Osmotic effects on the T2 relaxation decay of in vivo muscle
Osmotic effects on the T2 relaxation decay of in vivo muscle
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DOI：
10.1002/mrm.1232
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发表时间：
2001-09-01
影响因子：
3.3
通讯作者：
Mulkern, RV
中科院分区：
文献类型：
--
作者：
Gambarota, G;Cairns, BE;Mulkern, RV
生理盐水溶液通常用作肌肉内给药药物的载体。在这项研究中，在体内测量自旋-自旋弛豫（T-2）过程的磁共振成像（MRI）进行调查后，肌肉注射不同张力的生理盐水溶液的水在大鼠咬肌组织中的分布。在注射生理盐水之前，肌肉的基于图像的T-2弛豫衰减是单指数的。注射生理盐水后，T-2弛豫衰减变为多指数衰减。衰减曲线的非负最小二乘（NNLS）分析揭示了两个弛豫分量：快分量（T-2 = 20-40 ms）和慢分量（T-2 = 150-400 ms），其分别被分配给细胞内和细胞外水质子。注射高渗盐水溶液显着增加了细胞外的水成分在肌肉组织中的等渗盐水溶液相比，效果持续超过60分钟。这些研究结果表明，MRI技术可能是有用的，以调查高或低渗溶液对肌肉组织在体内的影响。(C)2001 Wiley-Liss，Inc.
Saline solutions are commonly employed as a vehicle for drugs administered intramuscularly. In this study, in vivo measurements of spin-spin relaxation (T-2) processes by magnetic resonance imaging (MRI) were performed to investigate the distribution of water in rat masseter muscle tissue after intramuscular injection of saline solutions of varying tonicity. Prior to saline injection, image-based T-2 relaxation decay of muscle was monoexponential. After injection of saline, the T-2 relaxation decay became multiexponential. Non-negative least squares (NNLS) analysis of the decay curves revealed two relaxation components: a fast component (T-2 = 20-40 ms) and a slow component (T-2 = 150-400 ms), which are assigned to intra- and extracellular water protons, respectively. Injection of hypertonic saline solutions significantly increased the extracellular water component in muscle tissue compared to isotonic saline solutions, an effect which lasted for more than 60 min. These findings suggest that MRI techniques may be useful to investigate the effect of hyper- or hypotonic solutions on muscle tissue in vivo. (C) 2001 Wiley-Liss, Inc.