Response of corneal epithelial cells to Staphylococcus aureus
Response of corneal epithelial cells to Staphylococcus aureus
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DOI：
10.4161/viru.1.4.11466
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发表时间：
2010-07-01
期刊：
影响因子：
5.2
通讯作者：
Gilmore, Michael S.
中科院分区：
文献类型：
--
作者：
Heimer, Susan R.;Yamada, Ai;Gilmore, Michael S.
金黄色葡萄球菌是侵袭性感染的主要原因。它还感染湿粘膜组织，包括角膜和结膜。Toll样受体在人角膜上皮细胞中的表达有明显的证据。因此，确定这种免疫豁免组织的上皮细胞如何对S.金黄色。此外，确定具有引起离子通量或潜在地允许效应分子移动穿过靶细胞膜的潜力的溶细胞毒素是否改变反应是有意义的。微阵列被用来全面评估人类角膜上皮细胞对S。金黄色。观察到编码抗微生物树突状细胞趋化因子CCL20的转录物丰度的大幅增加。检测到CCL20释放到培养基中，并且发现这种反应在很大程度上是TLR2和NOD2独立的。角膜上皮细胞也对S.金黄色葡萄球菌通过增加炎症介质、转录因子和MAP激酶途径相关基因的细胞内mRNA丰度，以类似于其他细胞类型的方式。令人惊讶的是，角膜上皮细胞反应不受毒素暴露的影响。然而，毒素暴露确实引起了应激反应。尽管S.金黄色葡萄球菌感染的阴道上皮细胞，获得的结果与报道的表达临床中毒性休克毒素的分离株刺激阴道上皮细胞的结果惊人地相似，表明最初的上皮细胞对S.金黄色葡萄球菌在很大程度上不依赖于菌株以及上皮细胞组织来源。
Staphylococcus aureus is a leading cause of invasive infection. It also infects wet mucosal tissues including the cornea and conjunctiva. Conflicting evidence exists on the expression of Toll-like receptors by human corneal epithelial cells. It was therefore of interest to determine how epithelial cells from this immune privileged tissue respond to S. aureus. Further, it was of interest to determine whether cytolytic toxins, with the potential to cause ion flux or potentially permit effector molecule movement across the target cell membrane, alter the response. Microarrays were used to globally assess the response of human corneal epithelial cells to S. aureus. A large increase in abundance of transcripts encoding the antimicrobial dendritic cell chemokine, CCL20, was observed. CCL20 release into the medium was detected, and this response was found to be largely TLR2 and NOD2 independent. Corneal epithelial cells also respond to S. aureus by increasing the intracellular abundance of mRNA for inflammatory mediators, transcription factors, and genes related to MAP kinase pathways, in ways similar to other cell types. The corneal epithelial cell response was surprisingly unaffected by toxin exposure. Toxin exposure did, however, induce a stress response. Although model toxigenic and non-toxigenic strains of S. aureus were employed in the present study, the results obtained were strikingly similar to those reported for stimulation of vaginal epithelial cells by clinical toxic shock toxin expressing isolates, demonstrating that the initial epithelial cellular responses to S. aureus are largely independent of strain as well as epithelial cell tissue source.