STABILITY OF SONICATED AQUEOUS SUSPENSIONS OF PHOSPHOLIPIDS UNDER AIR
STABILITY OF SONICATED AQUEOUS SUSPENSIONS OF PHOSPHOLIPIDS UNDER AIR
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DOI：
10.1016/0009-3084(91)90031-6
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发表时间：
1991-12-01
影响因子：
3.4
通讯作者：
SAMSONOFF, C
中科院分区：
文献类型：
--
作者：
ALMOG, R;FORWARD, R;SAMSONOFF, C
采用分光光度法、谷胱甘肽过氧化物酶反应性和薄层色谱法研究了脂质体水悬浮液中磷脂在空气中抗氧化降解的稳定性。发现两性离子磷脂易于通过多不饱和烃链的氧化和酯水解而降解，产生氧化溶血磷脂和游离脂肪酸衍生物。这些产品的特点是氢过氧化物的基础上与硒依赖性谷胱甘肽过氧化物酶分离的人红细胞的反应性。在Tris缓冲液中的卵磷脂比在水中更耐水解。8.0 mM大豆磷脂酰胆碱（SB-PC）在0.1 M Tris（pH 7.5）中的悬浮液在空气存在下的超声处理产生相对高浓度的共轭二烯过氧化氢，但少量的水解产物。阴离子磷脂，如卵磷脂酰甘油（egg-PG），表现出更高的抗空气氧化性比两性离子卵磷脂，但其氧化促进超声处理。
The stability of phospholipids in liposomal aqueous suspension against oxidative degradation in air was investigated using spectrophotometric indices, glutathione peroxidase reactivity and thin layer chromatography. Zwitterionic phospholipid was found to be susceptible to degradation via oxidation of polyunsaturated hydrocarbon chains and ester hydrolysis, producing oxidized lysophosphatide and free fatty acid derivatives. These products were characterized as hydroperoxides based on their reactivity with the selenium-dependent glutathione peroxidase isolated from human erythrocytes. Lecithin in Tris buffer was more resistant to hydrolysis than in water. The sonication of 8.0 mM of soybean phosphatidylcholine (SB-PC) suspension in 0.1 M Tris (pH 7.5) in the presence of air produced relatively high concentration of conjugated diene hydroperoxide, but a small amount of hydrolyzed products. Anionic phospholipids, such as egg-phosphatidylglycerol (egg-PG), demonstrated higher resistance to air oxidation than the zwitterionic lecithin, but its oxidation was promoted by sonication.