Detection of nitric oxide in macrophage cells for the assessment of the cytotoxicity of gold nanoparticles
Detection of nitric oxide in macrophage cells for the assessment of the cytotoxicity of gold nanoparticles
复制标题
检测巨噬细胞中的一氧化氮以评估金纳米颗粒的细胞毒性
DOI：
10.1016/j.talanta.2012.08.044
复制
发表时间：
2012-11-15
期刊：
影响因子：
6.1
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Du, Libo;Miao, Xiaoxiang;Liu, Yang
在本文中，使用一氧化氮传感器的电化学方法用于定量评价从金纳米粒子处理的巨噬细胞释放的NO。我们的研究结果表明，金纳米颗粒启动NO从巨噬细胞的释放和释放的量与金纳米颗粒的浓度呈正相关。同时，在金纳米粒子处理的巨噬细胞中的总亚硝酸盐/硝酸盐浓度已确定通过格里斯反应，我们表明，亚硝酸盐/硝酸盐浓度的变化是根据由电化学方法测量。与柠檬酸盐包被的金纳米颗粒（CT-AuNPs）相比，当AuNPs被巯基化聚乙二醇（PEG）保护时，巨噬细胞中NO的释放被缓解，这意味着PEG包被的AuNPs具有更低的细胞毒性和氧化应激潜力，这可能是由于其抑制了NO的产生。总之，这项工作已经证明了一个有效的传感平台，通过检测从巨噬细胞释放的细胞外NO的金纳米颗粒的细胞毒性的评估。(C)2012爱思唯尔有限公司版权所有。
In this paper, an electrochemical method using a nitric oxide sensor was employed for quantitative evaluation of NO released from AuNPs-treated macrophage cells. Our results indicate that the AuNPs initiate NO release from macrophage cells and the amount of NO released is positively correlated with concentration of AuNPs. Meanwhile, total nitrite/nitrate concentrations in the AuNPs-treated macrophage cells have been determined via the Griess reaction and we demonstrate that the variation of the nitrite/nitrate concentrations is in accordance with that measured by the electrochemical method. In contrast to the citrate-coated gold nanoparticles (CT-AuNPs), when AuNPs were protected by thiolated poly ethylene glycol (PEG), the NO-releasing in macrophage upon the addition of AuNPs was relieved, implying that the PEG-coated AuNPs having less cytotoxicity and oxidative stress potential is probably due to inhibition of NO production. In conclusion, this work has demonstrated an effective sensing platform for the evaluation of the cytotoxicity of AuNPs by detecting the extracellular NO released from macrophage cells. (C) 2012 Elsevier B.V. All rights reserved.