Quantitation of human thymus-leukemia-associated antigen in established hematopoietic cell lines by radioimmunoassay.
Quantitation of human thymus-leukemia-associated antigen in established hematopoietic cell lines by radioimmunoassay.
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通过放射免疫测定法对已建立的造血细胞系中的人胸腺白血病相关抗原进行定量。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
J. Minowada
中科院分区：
文献类型：
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作者：
B. Chechik;J. Minowada
应用竞争性放射免疫分析法测定了人胸腺白血病相关抗原（HTy-L）在白血病细胞系和正常造血细胞系中的含量。无论绵羊红细胞受体的存在与否，在所有检测的T细胞白血病细胞系中均检测到高度增加的HTy-L数量。这种升高的HTy-L水平与高末端脱氧核苷酸转移酶和腺苷脱氨酶活性的组合以及T淋巴细胞特异性表面抗原的存在似乎代表了T细胞系的稳定表型特征。大多数正常B细胞系具有低量的HTy-L。在相当数量的淋巴瘤B细胞系和所有测试的非T、非B白血病细胞系中，HTy-L的水平略微升高。在B细胞系中HTy-L的数量和不同表面免疫球蛋白同种型的表达之间不存在关系。在白血病髓样细胞和骨髓瘤细胞系以及源自B细胞急性淋巴细胞白血病患者的B细胞白血病细胞系中检测到少量的HTy-L。显然，T细胞白血病细胞系中HTy-L数量的增加可能与发生白血病细胞转化的T细胞分化的某些阶段有关。
A competitive radioimmunoassay for a saline-soluble human thymus-leukemia-associated antigen (HThy-L) was applied for quantitation of this antigen in leukemia and normal hematopoietic cell lines. Highly increased quantities of HThy-L were detected in all T-cell leukemia lines tested, regardless of the presence or absence of receptors for sheep erythrocytes. This elevated level of HThy-L in combination with high terminal deoxynucleotidyl transferase and adenosine deaminase activities and the presence of a T-lymphocyte-specific surface antigen appear to represent stable phenotypic characteristics of T-cell lines. Most normal B-cell lines had low quantities of HTy-L. The level of HThy-L was slightly elevated in a considerable number of lymphoma B-cell lines and in all non-T, non-B leukemia cell lines tested. No relationship existed between quantities of HThy-L and an expression of different surface immunoglobulin isotypes in B-cell lines. Low quantities of HThy-L were detected in leukemia myeloid and myeloma cell lines as well as in B-cell leukemia lines originating from patients with B-cells acute lymphoblastic leukemia. Apparently, the increased quantities of HThy-L in T-cell leukemia lines may be related to certain stages of T-cell differentiation at which leukemia cell transformation occurs.