NICOTINE-EVOKED DISASSEMBLY OF CORTICAL ACTIN-FILAMENTS IN ADRENAL CHROMAFFIN CELLS
NICOTINE-EVOKED DISASSEMBLY OF CORTICAL ACTIN-FILAMENTS IN ADRENAL CHROMAFFIN CELLS
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DOI：
10.1016/0014-5793(86)80022-9
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发表时间：
1986-10-20
期刊：
影响因子：
3.5
通讯作者：
BURGOYNE, RD
中科院分区：
文献类型：
--
作者：
CHEEK, TR;BURGOYNE, RD
用罗丹明-鬼笔环肽染色，发现肌动蛋白丝集中在休息嗜铬细胞的皮质。皮质肌动蛋白丝在尼古丁刺激后15秒解体，30秒后重新组装。肌动蛋白丝解体后，烟碱刺激也检测使用DNase I抑制试验。钙依赖于外部钙，对三氟拉嗪不敏感，高K+、毒蕈碱激动剂或佛波酯不引起钙依赖。皮层肌动蛋白丝的断裂可能允许分泌颗粒进入胞吐位点，并与细胞内游离钙的升高一起作用，从而引起烟碱激动剂引起的完全分泌反应。
Using rhodamine-phalloidin staining it was found that actin filaments are concentrated in the cortex of resting chromaffin cells. Cortical actin filaments were disassembled 15 s after stimulation by nicotine and had reassembled 30 s later. Actin filament disassembly following nicotinic stimulation was also detected using the DNase I inhibition assay. Disassembly was independent of external calcium, insensitive to trifluoperazine and was not elicited by high K+, muscarinic agonists or phorbol ester. Disassembly of cortical actin filaments may allow access of secretory granules to exocytotic sites and act in conjunction with a rise in intracellular free calcium to bring about the full secretory response due to nicotinic agonists.