HIGH-ACCURACY MOLECULAR MASS DETERMINATION OF PROTEINS USING MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY
HIGH-ACCURACY MOLECULAR MASS DETERMINATION OF PROTEINS USING MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY
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DOI：
10.1021/ac00216a020
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发表时间：
1990-09-01
影响因子：
7.4
通讯作者：
CHAIT, BT
中科院分区：
文献类型：
--
作者：
BEAVIS, RC;CHAIT, BT
一种获得蛋白质分子量的方法，其精确度约为±。0.01%的基质辅助激光解吸使用内部校准。该技术允许精确的蛋白质样品大小为1 pmol的质量测定。高浓度的有机和无机污染物（例如1 M尿素）不会强烈影响信号强度或质量分配。为蛋白质分配准确分子量的能力取决于在质谱中观察到的清晰分辨的质子化分子离子。
A method for obtaining protein molecular masses with an accuracy of approximately .+-. 0.01% by matrix-assisted laser desorption using an internal calibrant is described. The technique allows accurate mass determinations of protein sample sizes as small as 1 pmol. High concentrations of organic and inorganic contaminants (e.g. 1 M urea) do not strongly affect either the signal intensity or the mass assignment. The ability to assign an accurate molecular mass to a protein is contingent on the observation of clearly resolved protonated molecule ions in the mass spectrum.