A high-throughput histoarray for quantitative molecular profiling of multiple, uniformly oriented medaka (Oryzias latipes) embryos
A high-throughput histoarray for quantitative molecular profiling of multiple, uniformly oriented medaka (Oryzias latipes) embryos
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DOI：
10.1016/j.cbpc.2011.05.010
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发表时间：
2012-01-01
影响因子：
3.9
通讯作者：
Au, Doris W. T.
中科院分区：
文献类型：
--
作者：
Cheung, Napo K. M.;Hinton, David E.;Au, Doris W. T.
水生动物模式鱼的胚胎已被证明是发育生物学和早期生命阶段毒性试验的有用生物。由于其透明的绒毛膜，可以检测到正常和异常发育的成像，并与暴露或环境因素引起的变化有关。然而，在组织的亚个体水平的变化的检测受到阻碍的时间需要检测受影响的生物体的细胞和组织内的重要事件。我们在此描述了一种高成本效益的胚胎芯片的开发，该芯片能够在胚胎和eleutheroembryos中进行严格的个体间比较和多重检测。作为一个原则的证明，我们检查细胞增殖和控制细胞死亡在常氧和缺氧条件下，并与这些组织在个别生物体的营业额。再加上最近开发的整个成年动物平台，我们现在可以超越专注于单个靶器官的常见方法，检测和表征影响生物体的系统性现象（综合征）。综上所述，我们现在可以确定早期生命阶段暴露的成年后果，并评估暴露个体对沿着时间轴叠加的压力的反应能力。(C)2011 Elsevier Inc. All rights reserved.
Embryos of aquatic animal model fish have proven to be useful organisms for developmental biology and for early life stage toxicity tests. By virtue of their transparent chorions, imaging of normal and abnormal development can be detected and related to exposure or to alterations due to environmental factors. However, the detection of changes at sub-individual levels of organization is hampered by time required to detect important events within cells and tissues of affected organisms. We describe herein development of a highly cost effective embryo chip enabling stringent inter-individual comparisons and multiplex detection in embryos and eleutheroembryos. As a proof of principle we examine cell proliferation and controlled cell death in normoxic and hypoxic conditions and relate these to tissue turnover in individual organisms. Coupled with a recently developed whole adult animal platform, we can now move beyond the common approach focusing on single target organ to the detection and characterization of systemic phenomena (syndromes) affecting the organism. Taken together, we can now determine adult consequences of early life stage exposure and assess ability of exposed individuals to respond to stresses superimposed along the axis of time. (C) 2011 Elsevier Inc. All rights reserved.