Estradiol and tamoxifen reverse ovariectomy-induced physical inactivity in mice
Estradiol and tamoxifen reverse ovariectomy-induced physical inactivity in mice
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DOI：
10.1249/01.mss.0000241649.15006.b8
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发表时间：
2007-02-01
期刊：
影响因子：
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通讯作者：
Lowe, Dawn A.
中科院分区：
文献类型：
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作者：
Gorzek, Jeffrey F.;Hendrickson, Kaysie C.;Lowe, Dawn A.
身体活动减少和体重增加与雌激素缺乏有关。目的：确定雌激素或雌激素类似物三苯氧胺是否可以逆转小鼠卵巢切除术后的这些不利影响。方法：10周龄C57 BL/6小鼠假手术（sham，N = 6）或卵巢切除（OVX，N = 9）。在自愿轮跑4周后，植入安慰剂（OVX-P）或17 β-雌二醇（OVX-E-2）丸粒，并且小鼠再跑4周。第二项研究中，小鼠接受安慰剂，17 β-雌二醇，或他莫昔芬（OVX-Tam）同时卵巢切除术。每24小时跑的距离和体重通过重复测量的双因素方差分析进行分析。结果如下：在最初的4周内，OVX小鼠与假手术小鼠相比，跑步减少了约80%，体重增加了约20%（P < 0.001）。随着OVX-E-2小鼠从1.9 +/- 0.3 km（.）24 h（-1）至6.9 +/- 0.7 km，相当于假手术（8.1 +/- 0.7 km），而OVX-P小鼠仅跑0.5 +/- 0.2 km（P < 0.01）。OVX-E-2小鼠在雌二醇替代后倾向于保持体重，而OVX-P小鼠继续增加体重。接受他莫昔芬的OVX小鼠具有高跑步活动，约9公里。24 h（-1），维持体质量。结论：卵巢激素的去除导致小鼠变得不活跃，体重增加。以17 β-雌二醇或他莫昔芬形式的激素治疗迅速刺激自愿轮跑步并逆转体重增加，表明雌激素受体结合参与调节体力活动。
Decreased physical activity and increased body mass are associated with estrogen deficiency. Purpose: To determine whether estrogen or the estrogen analog, tamoxifen, could reverse those detrimental effects after surgical ovariectomy in mice. Methods: Ten-week-old C57BL/6 mice were sham operated (sham, N = 6) or ovariectomized (OVX, N = 9). After 4 wk of voluntary wheel running, placebo (OVX-P) or 17 beta-estradiol (OVX-E-2) pellets were implanted and the mice ran an additional 4 wk. A second study followed in which mice received placebo, 17 beta-estradiol, or tamoxifen (OVX-Tam) simultaneously with ovariectomies. Distances run per 24 h and body masses were analyzed by two-way ANOVA with repeated measures. Results: During the initial 4 wk, OVX mice ran approximately 80% less and had approximately 20% greater body masses compared with sham mice (P < 0.001). Estradiol replacement quickly reversed the inactivity as OVX-E-2 mice increased their running from 1.9 +/- 0.3 km(.)24 h(-1) to 6.9 +/- 0.7 kin within a week of replacement, which was equivalent to shams (8.1 +/- 0.7 km), whereas OVX-P mice ran only 0.5 +/- 0.2 knit (P < 0.01). OVX-E-2 mice tended to maintain body mass after estradiol replacement, whereas the OVX-P mice continued to increase mass. OVX mice that received tamoxifen had high running activity, approximately 9 km(.)24 h(-1), and maintained body mass. Conclusion: The removal of ovarian hormones caused mice to become inactive and gain body mass. Hormone therapy in the form of 17 beta-estradiol or tamoxifen rapidly stimulated voluntary wheel running and reversed body mass gains, indicating that estrogen receptor binding was involved in regulating physical activity.