Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
复制标题
高原藏族慢性鼻窦炎的免疫病理学特征
DOI：
10.2500/ar.2013.4.0055
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Bachert C
中科院分区：
文献类型：
--
作者：
Luo B;Feng L;Jintao D;Yafeng L;Shixi L;Nan Z;Bachert C
慢性鼻窦炎（CRS）在不同人群和环境中表现出不同的炎症和重塑模式。藏族生活在高海拔和寒冷的气候条件下。我们试图研究藏人是否表现出独特的CRS病理或特征。鼻息肉和粘膜组织取自14例藏族CRS伴鼻息肉患者（CRSwNPs）、13例CRS不伴鼻息肉患者（CRSsNPs）和12例藏族对照。组织匀浆和血清样品进行了测定几个辅助性T细胞（TH）细胞因子和介质使用酶联免疫吸附试验的配置文件，采用定量聚合酶链反应。对几种关键的炎症细胞进行免疫组织化学标记物检查。CRSwNP的特征在于促进嗜酸性粒细胞炎症的介质（白细胞介素[IL]-5、嗜酸性粒细胞阳离子蛋白和总免疫球蛋白E）增加，与IL-8、IL-2sRa、IL-1 β、IL-6和髓过氧化物酶的表达有轻微的协同作用，嗜酸性粒细胞、肥大细胞和中性粒细胞占优势。与对照组相比，CRSwNPs中加塔-3转录因子显著增加，Foxp 3表现出受损的趋势。与CRSwNP相比，CRSsNP的特征在于显著高水平的转化生长因子β 1、增加的干扰素γ以及Foxp 3和T-β的显著增强。CRSsNP中炎性细胞数量减少，但巨噬细胞水平增加。与CRSsNP相比，CRSwNP呈现严重的炎症反应，并显示具有明显受损的调节性T细胞（Treg）功能和增加的以嗜酸性粒细胞和肥大细胞为主的炎性细胞浸润的TH 2环境。相反，具有增强的Treg功能和增加的巨噬细胞水平的TH 1极化出现在CRSsNP中。
Chronic rhinosinusitis (CRS) presents distinct inflammatory and remodeling patterns in different populations and environments. Tibetan ethnic groups live at high altitudes and in cold weather conditions. We sought to examine whether Tibetans exhibit distinct CRS pathology or characteristics. Sinonasal polyps and mucosal tissue were obtained from 14 Tibetan patients with CRS and nasal polyps (CRSwNPs), 13 patients with CRS without nasal polyps (CRSsNPs), and 12 Tibetan controls. Tissue homogenates and serum samples were assayed for several T-helper (TH) cell cytokines and mediators using enzyme linked immunosorbent assay profiles were measured using quantity polymerase chain reaction. Several key inflammatory cells were examined for immunohistochemical markers. CRSwNPs were characterized by increased mediator promoting eosinophilic inflammation (interleukin [IL]-5, eosinophil cationic protein, and total immunoglobulin E) and slight synergism with expression of IL-8, IL-2sRa, IL-1beta, IL-6, and myeloperoxidase, and a predominance of eosinophils, mast cells, and neutrophils. GATA-3 transcription factor was significantly increased and Foxp3 showed a tendency to be impaired in CRSwNPs compared with controls. CRSsNPs were characterized by significantly high levels of transforming growth factor beta1, increased interferon γ, and a significant enhancement of Foxp3 and T-beta compared with CRSwNPs. There were reduced numbers of inflammatory cells but increased levels of macrophages in CRSsNPs. Compared with CRSsNPs, CRSwNPs present a severe inflammatory reaction and show a TH2 milieu with apparently impaired regulatory T cells (Treg) function and increased inflammatory cells infiltration predominated by eosinophilic and mast cells. In contrast, TH1 polarization with enhanced Treg function and increased levels of macrophages appear in CRSsNPs.