Amperometric Biosensor Based on Galactose Oxidase Immobilized in Clay Matrix
Amperometric Biosensor Based on Galactose Oxidase Immobilized in Clay Matrix
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DOI：
10.1002/elan.201200274
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发表时间：
2013-03-01
期刊：
影响因子：
3
通讯作者：
Mousty, Christine
中科院分区：
文献类型：
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作者：
Charmantray, Franck;Touisni, Nadia;Mousty, Christine
通过将半乳糖氧化酶 (GAOx) 固定在涂在 Pt 电极表面的合成锂皂石粘土膜内，开发了一种电流半乳糖生物传感器。通过将 GAOx/合成锂皂石生物电极相对于 Ag/AgCl 的电位稳定在 +0.6V 来进行电化学测定，以氧化酶促产生的过氧化氢。生物传感器的生物电化学响应在缓冲液性质、pH 值和 GAOx/锂皂石比率方面进行了优化。 Laponite 为 GAOx 活性提供了有利的环境，因为优化的生物传感器 GAOx/laponite（50:25；mu g:mu g）在 1.0x1061.6x103M 的线性半乳糖浓度范围内表现出极高的灵敏度和最大电流（分别为 85mA M1 cm2 和 232 mu A cm2）。它在 4°C 下至少可稳定四个星期。
An amperometric galactose biosensor was developed by immobilizing galactose oxidase (GAOx) within a laponite clay film coated on a Pt electrode surface. The electrochemical assays were performed by potentiostating the GAOx/laponite bioelectrodes at +0.6V vs. Ag/AgCl in order to oxidize the enzymatically generated hydrogen peroxide. The bioelectrochemical responses of the biosensors were optimized with regard to the buffer nature, the pH, and the GAOx/laponite ratios. Laponite provides a favorable environment to GAOx activity, since the optimized biosensor GAOx/laponite (50:25; mu g:mu g) shows a great sensitivity and maximum current (85mA M1 cm2 and 232 mu A cm2, respectively) over a linear galactose concentration range of 1.0x1061.6x103M. It is also stable at least for four weeks at 4 degrees C.