Comparison of the efficacy of Neethling lumpy skin disease virus and x10RM65 sheep-pox live attenuated vaccines for the prevention of lumpy skin disease - The results of a randomized controlled field study
Comparison of the efficacy of Neethling lumpy skin disease virus and x10RM65 sheep-pox live attenuated vaccines for the prevention of lumpy skin disease - The results of a randomized controlled field study
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DOI：
10.1016/j.vaccine.2015.07.071
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发表时间：
2015-09-11
期刊：
影响因子：
5.5
通讯作者：
Shpigel, N. Y.
中科院分区：
文献类型：
--
作者：
Ben-Gera, J.;Klement, E.;Shpigel, N. Y.
肿块性皮肤病 (LSD) 是牛和水牛的一种病毒性疾病，由羊痘病毒引起。在 2012 年至 2013 年以色列发生 LSD 流行期间进行了一项实地研究，以评估两种商业疫苗预防 LSD 的功效。 15 个奶牛群参加了这项研究，它们在研究开始前 2-5 个月接种了单剂 10(2.5) TCID50 的 RM65 减毒羊痘疫苗，并且之前未受到影响。 4694 头奶牛被随机分为接种 10(3.5) TCID50/剂量的 RM65 疫苗 (x10RM65) 或接种相同剂量的 Neethling LSD 病毒减毒疫苗。 LSD 病例被定义为出现至少 5 个 LSD 典型病变，如果该体征伴有发烧（>39.5 摄氏度）或/和产奶量减少 20%，则定义为严重病例。从64.5%的病例中采集了深层病灶活检和血液样本，试图通过PCR检测LSD病毒的DNA，并区分野生株和疫苗Neethling株。 8 个牛群的 76 头奶牛受到 LSD 影响，发病率为 0.3-5.7%。 x10RM65 与 Neethling 疫苗接种后至少 15 天的 Mantel-Haenszel 相对风险 (RRMH) 为 2.635 (CI95% = 1.44-4.82)，严重发病为 11.2(2.3-54.7)。实验室确诊病例的 RMMH 为 4.28 (1.59-11.53)。在接种 Neethling 疫苗的奶牛中观察到 Neethling 相关疾病的发生率为 0.38% (9/2356)，而在 x10RM65 接种疫苗的奶牛中没有发生此类疾病。我们的结论是，Neethling 疫苗在预防 LSD 发病方面比 x10RM65 显着更有效，尽管它可能会降低 Neethling 相关疾病的发病率。本研究中未观察到 Neethling 菌株向未接种 Neethling 疫苗的奶牛传播。 (C) 2015 Elsevier Ltd. 保留所有权利。
Lumpy skin disease (LSD) is a viral disease of cattle and buffalo, caused by a Capripox virus. A field study was performed during an LSD epidemic which occurred in 2012-2013 in Israel, in order to assess the efficacy of two commercial vaccines for protection against LSD. Fifteen dairy herds, vaccinated 2-5 months prior to study onset with a single dose of 10(2.5) TCID50 of RM65 attenuated sheep-pox vaccine, and not affected previously, were enrolled in the study. 4694 cows were randomized to be either vaccinated with a 10(3.5) TCID50/dose of RM65 vaccine (x10RM65) or with a same dose of an attenuated Neethling LSD virus vaccine. A case of LSD was defined as the appearance of at least 5 lesions typical to LSD and a severe case was defined if this sign was accompanied by either fever (>39.5 degrees C) or/and a 20% reduction in milk production. Deep lesion biopsies and blood samples were collected from 64.5% of the cases in an attempt to detect DNA of LSD virus by PCR and to differentiate between the wild strain and the vaccine Neethling strain. Seventy-six cows were affected by LSD in 8 herds with an incidence of 0.3-5.7%. Mantel-Haenszel relative risk (RRMH) for LSD morbidity at least 15 days after vaccination in x10RM65 vs. Neethling was 2.635 (CI95% = 1.44-4.82) and 11.2(2.3-54.7) for severe morbidity. RRMH for laboratory confirmed cases was 4.28 (1.59-11.53). An incidence of 0.38% (9/2356) of Neethling associated disease was observed among Neethling vaccinated cows while no such disease occurred in x10RM65 vaccinated cows. We conclude that the Neethling vaccine is significantly more effective than x10RM65 in preventing LSD morbidity, though it might cause a low incidence of Neethling associated disease. No transmission of the Neethling strain to non-Neethling vaccinated cows was observed in this study. (C) 2015 Elsevier Ltd. All rights reserved.