Mast cells, eosinophils and antibody-mediated cellular cytotoxicity are not critical in resistance to Trichuris muris
Mast cells, eosinophils and antibody-mediated cellular cytotoxicity are not critical in resistance to Trichuris muris
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DOI：
10.1046/j.1365-3024.1999.00200.x
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发表时间：
1999-01-01
影响因子：
2.2
通讯作者：
Else, KJ
中科院分区：
文献类型：
--
作者：
Betts, CJ;Else, KJ
小鼠肠道线虫Trichuris muris为人类感染鞭虫提供了一个非常有价值的模型，因此，分析小鼠的免疫应答可能有助于阐明人类对鞭虫的免疫机制。从宿主肠道中分离出来在去除IL-5后，因此嗜酸性粒细胞增多，小鼠通常对T。小鼠感染仍然如此。此外，阻断干细胞因子受体，c-kit，以促进在抵抗小鼠的寄生虫排出期间完全消融肥大细胞，对保护性免疫的发展没有影响。因此，可以推断嗜酸性粒细胞和肥大细胞在抵抗中不是关键的。除这些研究外，还通过分析Fc γ R-/-小鼠中的感染时程研究了抗体介导的细胞毒性机制的作用。这些动物在抗性背景下完全免疫，并在成虫阶段发育之前排出寄生虫。因此，该模型为ADCC在抵抗T.本文描述的研究已经消除了传统上与蠕虫感染相关的一些主要效应机制，并且继续致力于阐明与抗性相关的关键免疫应答。
The murine intestinal nematode Trichuris muris provides an invaluable model of human infection with T, trichiura, Hence, analysis of the immunological responses in the mouse may elucidate the mechanisms of immunity to trichuriasis in man. The work described here investigates the roles of eosinophils, mast cells and antibody-dependent cell-mediated cytotoxicity (ADCC) in the elimination of T. muris from the host gut. Following ablation of IL-5, and hence eosinophilia, mice usually resistant to T. muris infection remained so. Further, blocking the stem cell factor receptor, c-kit, to facilitate complete ablation of mast cells over the period of parasite expulsion in resistant mice had no effect on the development of protective immunity. Therefore it can be deduced that eosinophils and mast cells are not critical in resistance. In addition to these studies, the role of antibody-mediated cellular cytotoxic mechanisms was investigated via the analysis of an infection time course in Fc gamma R-/- mice. These animals, on a resistant background, were fully immune and expelled the parasites before development of the adult stage. Thus this model provides evidence against a major role for ADCC in resistance to infection with T. muris, The studies described here have eliminated some of the major effector mechanisms traditionally associated with helminth infection, and work continues to elucidate the critical immune responses associated with resistance.