Spatio-temporal remodeling of extracellular matrix orients epithelial sheet folding
Spatio-temporal remodeling of extracellular matrix orients epithelial sheet folding
复制标题
细胞外基质的时空重塑定向上皮片折叠
DOI：
10.1101/2023.01.13.523870
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kondo Takefumi
中科院分区：
文献类型：
--
作者：
Tsuboi Alice;Fujimoto Koichi;Kondo Takefumi
生物系统固有的噪音，但是，他们产生高度定型的组织morphology.Drosophilapupal翅膀显示了高度定型的折叠，通过均匀的扩张和随后的屈曲翅上皮细胞在周围的角质层囊。屈曲产生的折叠模式通常是随机的;因此不清楚屈曲如何导致翅膀的定型组织折叠。我们发现，细胞外基质（ECM）蛋白质，矮胖，指导的位置和方向的屈曲诱导的褶皱。矮胖将翅上皮固定在特定组织位置的上覆角质层上。组织范围内的改变Dumpy沉积和降解产生了不同的屈曲模式。总之，我们提出，时空ECM重塑形状刻板的组织折叠通过上皮和其外部结构之间的动态相互作用。
Biological systems are inherently noisy; however, they produce highly stereotyped tissue morphology.Drosophilapupal wings show a highly stereotypic folding through uniform expansion and subsequent buckling of wing epithelium within a surrounding cuticle sac. The folding pattern produced by buckling is generally stochastic; it is thus unclear how buckling leads to stereotypic tissue folding of the wings. We found that the extracellular matrix (ECM) protein, Dumpy, guides the position and direction of buckling-induced folds. Dumpy anchors the wing epithelium to the overlying cuticle at specific tissue positions. Tissue-wide alterations of Dumpy deposition and degradation yielded different buckling patterns. In summary, we propose that spatiotemporal ECM remodeling shapes stereotyped tissue folding through dynamic interactions between the epithelium and its external structures.