Participation of the recA determinant in the transposition of class II transposon mini-TnMERI1.
Participation of the recA determinant in the transposition of class II transposon mini-TnMERI1.
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DOI：
10.1016/j.femsle.2005.09.053
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发表时间：
2005-12
影响因子：
2.1
通讯作者：
K. Matsui;M. Narita;H. Ishii;G. Endo
中科院分区：
文献类型：
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作者：
K. Matsui;M. Narita;H. Ishii;G. Endo
作为理解II类汞抗性转座子TnMERI 1的移动的性质的初始步骤，评价了therecA基因对mini-TnMERI 1易位的影响。LE 392株的转座频率（2.4 ± 1.2 × 10−5）高于recA缺陷型DH 1株（1.2 ± 0.8 × 10−6），表明recA基因参与了mini-TnMERI 1转座。将therecA基因引入DH 1菌株中补充了与LE 392相同水平的转座频率，并证实了therecA基因参与转座。然而，用应激剂处理细胞，包括高达3000 J m−2UV剂量的照射，并没有改变转座频率，表明RecA与SOS应激反应无关。进一步分析transconjugants表明参与RecA的决议的共整合结构的转座子。这些结果表明，RecA是一个组成性的细胞因子，增加了小TnMERI 1的易位，并可能参与TnMERI 1样转座子的传播。
As an initial step to understand the mobile nature of class II mercury resistance transposon TnMERI1, the effect of therecAgene on translocation of mini-TnMERI1was evaluated. A higher transposition frequency in the LE392 strain (2.4 ± 1.2 × 10−5) than in therecA-deficient DH1 strain (1.2 ± 0.8 × 10−6) indicated participation of therecAgene in mini-TnMERI1transposition. Introduction of therecAgene into the DH1 strain complemented the transposition frequency at the same level as in LE392 and confirmed participation of therecAgene in transposition. However, treatment of cells by stress agents, including irradiation of up to 3000 J m−2UV doses, did not alter the transposition frequency and suggested independence of RecA from the SOS stress response. Further analysis of transconjugants indicated participation of RecA in the resolution of the cointegrate structure of the transposon. These results suggested that RecA is a constitutive cellular factor that increases translocation of mini-TnMERI1and may participate in dissemination of TnMERI1-like transposons.