Metabolic correlates of intracellular pH change during rapid contractile activity in a molluscan muscle
Metabolic correlates of intracellular pH change during rapid contractile activity in a molluscan muscle
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软体动物肌肉快速收缩活动期间细胞内 pH 变化的代谢相关性
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
W. Ellington
中科院分区：
文献类型：
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作者：
C. Chih;W. Ellington
利用31 P-NMR技术研究了海湾扇贝（Argopecten irradians concentricus）闭壳肌爆发性收缩过程中细胞内pH的变化。在收缩的第一阶段观察到短暂的碱化（从pHi 7.06到7.15）。在此期间，三磷酸腺苷（ATP）生产的主要模式是通过精氨酸磷酸的水解，这是一个消耗质子的过程。随后的肌肉收缩导致逐渐酸化，pHi接近6.94。在这些收缩的后期阶段，章鱼碱的糖酵解产物是ATP的主要来源。根据磷酸精氨酸利用的质子消耗和章鱼碱生产的质子产率，估计了能量转化的净质子产率。由此产生的质子变化的预测模式是非常相似的观察到的变化pH值。收缩活动过程中细胞内pH的变化。集中的辐合肌相位内收肌与发生在组织中的代谢能量转换的质子产率模式密切相关。
Intracellular pH (pHi) changes during burst contractile activity in in vitro preparations of phasic adductor muscle of the bay scallop, Argopecten irradians concentricus, were monitored using phosphorus nuclear magnetic resonance (31P-NMR) spectroscopy. A transient alkalinization (from pHi 7.06 to 7.15) was observed during the first period of contractions. The predominant mode of adenosine triphosphate (ATP) production during this period was by hydrolysis of arginine phosphate, a proton consuming process. Subsequent contractions of the muscle resulted in a gradual acidification, with pHi approaching 6.94. During these later stages of contraction, glycolytic production of octopine was a major source of ATP. The net proton yields of energy transformations were estimated, based on proton consumption by arginine phosphate utilization and proton yield of octopine production. The resulting predicted pattern of proton change was very similar to those of observed changes in pHi. Intracellular pH changes during contractile activity in A. irradians concentricus phasic adductor muscle are strongly correlated with the pattern of proton yield of metabolic energy transformations taking place in the tissue.
骨骼肌纤维疲劳。
DOI：
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发表时间：
1983
期刊：
Progress in clinical and biological research
影响因子：
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作者：
Faulkner,JA
通讯作者：
Faulkner,JA