Cloning and characterization of the human type II arginase gene.
Cloning and characterization of the human type II arginase gene.
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DOI：
10.1006/geno.1996.0606
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发表时间：
1996-12
期刊：
影响因子：
4.4
通讯作者：
J. Vockley;C. Jenkinson;Hridayabiranjan Shukla;R. Kern;W. Grody;S. Cederbaum
中科院分区：
文献类型：
--
作者：
J. Vockley;C. Jenkinson;Hridayabiranjan Shukla;R. Kern;W. Grody;S. Cederbaum
人体中有两种形式的精氨酸酶，都催化精氨酸水解为鸟氨酸和尿素。最近在动物模型和I型精氨酸酶缺陷患者中的研究表明，II型精氨酸酶是诱导型的，并且可能通过调节细胞精氨酸浓度在调节尿素循环外精氨酸代谢中发挥重要作用。我们PCR扩增并克隆了人Ⅱ型谷胱甘肽转移酶基因，这是第一个在哺乳动物中报道的非肝谷胱甘肽转移酶基因。虽然与I型丝氨酸蛋白酶的序列同源性、丝氨酸蛋白酶活性数据和用抗AII抗体的免疫沉淀证实了该基因的同一性，但北方印迹分析证实了其在脑、前列腺和肾中的差异表达。II型精氨酸酶可能是精氨酸调节系统的重要组成部分，影响一氧化氮合酶、精氨酸脱羧酶、kyotorphin合酶和精氨酸-甘氨酸转氨酶活性以及多胺和脯氨酸生物合成。
There are two forms of arginase in humans, both catalyzing the hydrolysis of arginine to ornithine and urea. Recent studies in animal models and in Type I arginase-deficient patients suggest that Type II arginase is inducible and may play an important role in the regulation of extra-urea cycle arginine metabolism by modulating cellular arginine concentrations. We PCR amplified and cloned the human Type II arginase gene, the first nonliver arginase gene reported in mammals. While sequence homology to Type I arginase, arginase activity data, and immunoprecipitation with an anti-AII antibody confirm the identity of this gene, Northern blot analysis demonstrates its differential expression in the brain, prostate, and kidney. Type II arginase may be an important part of the arginine regulatory system affecting nitric oxide synthase, arginine decarboxylase, kyotorphin synthase, and arginine-glycine transaminase activities and polyamine and proline biosynthesis.