Structure of the Eip28/29 gene, an ecdysone-inducible gene from Drosophila.
Structure of the Eip28/29 gene, an ecdysone-inducible gene from Drosophila.
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Eip28/29 基因的结构，果蝇的蜕皮激素诱导基因。
DOI：
10.1016/0022-2836(86)90492-4
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发表时间：
1986
影响因子：
5.6
通讯作者：
Cherbas,P
中科院分区：
文献类型：
--
作者：
Cherbas,L;Schulz,RA;Koehler,MM;Savakis,C;Cherbas,P
EIP 28和29是最初通过其在果蝇细胞系中的蜕皮激素诱导物鉴定的多肽家族。至少有两个家族成员，28 III和29 III，似乎是主要的翻译产物。在这里，我们描述了一个独特的Eip 28/29基因，必须编码两个初级产品。Eip 28/29基因是独特的，因为克隆的基因组DNA在严格条件下与EIP 28和29信使RNA杂交，但即使在温和条件下也不会与其他基因组序列可检测地退火。此外，该基因的不同产物不是等位基因，因为含有Eip 28/29基因的染色体区域（71 CD）纯合的果蝇产生翻译产生所有EIP 28和29的mRNA。通过与互补DNA序列的比较和核酸酶保护实验，我们推导出了Eip 28/29转录单位的完整结构。初级转录物长2146个核苷酸，并通过去除三个内含子进行加工，以在组织培养细胞中产生主要的成熟转录物（979个核苷酸）。该转录本可能对应于28 III mRNA。无论是转录单位的起始还是主要转录物的结构都不受激素蜕皮激素的影响。基因组序列揭示了一系列未知功能的七核苷酸和八核苷酸重复序列，这些重复序列在转录单位上游的前150个核苷酸内以约50个核苷酸的间隔下降。此外，当与先前发表的数据结合时，该序列表明果蝇中的共有帽位点序列可以扩展到包括以Snyderet等人（1982）先前识别的七核苷酸核心为中心的13个核苷酸。
The EIPs 28 and 29 are a family of polypeptides identified originally by their ecdysone inducibility inDrosophilacell lines. At least two family members, 28III and 29III, appear to be primary translation products. Here we describe a uniqueEip28/29 gene that must encode both primary products. TheEip28/29 gene is unique because the cloned genomic DNA hybridizes to both EIP 28 and 29 messenger RNAs under stringent conditions, but does not anneal detectably to other genomic sequences even under mild conditions. Furthermore the diverse products of this gene are not alleles because flies homozygous for the chromosomal region (71CD) containing theEip28/29 gene produce mRNAs that translate to yield all the EIPs 28 and 29.We report here the sequence of a 2855-nucleotide region encompassing theEip28/29 gene. By comparisons with complementary DNA sequences and by nuclease protection experiments we have derived a complete structure for theEip28/29 transcription unit. The primary transcript is 2146 nucleotides long and is processed by the removal of three introns to yield the predominant mature transcript in tissue culture cells (979 nucleotides). This transcript probably corresponds to the 28III mRNA. Neither the start of the transcription unit nor the structure of the predominant transcript is affected by the hormone ecdysone.The genomic sequence reveals a series of heptanucleotide and octanucleotide repeats of unknown function that fall at about 50-nucleotide intervals within the first 150 nucleotides upstream from the transcription unit. In addition this sequence, when combined with previously published data, suggests that the consensus cap site sequence inDrosophilamay be extended to include 13 nucleotides centered on the heptanucleotide core previously recognized by Snyderet al. (1982).