Diffusion-weighted magnetic resonance imaging reflects activation of signal transducer and activator of transcription 3 during focal cerebral ischemia/reperfusion.
Diffusion-weighted magnetic resonance imaging reflects activation of signal transducer and activator of transcription 3 during focal cerebral ischemia/reperfusion.
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DOI：
10.4103/1673-5374.211192
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发表时间：
2017-07
影响因子：
6.1
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Wu WJ;Jiang CJ;Zhang ZY;Xu K;Li W
信号转导和转录激活因子（Signaltransducerandactivatoroftranscription，STAT）是一个独特的蛋白质家族，与DNA结合，与酪氨酸磷酸化信号通路偶联，作为转录调节因子介导多种生物学效应。脑缺血再灌注可激活STAT信号通路，但目前尚无研究证实脑缺血再灌注后STAT激活是否可通过弥散加权磁共振成像（DWI）证实。本文采用改良Longa法建立大鼠局灶性脑缺血损伤模型。DWI显示再灌注前左半球部分区域高信号和低表观扩散系数。再灌注后STAT 3蛋白表达无明显变化，但磷酸化STAT 3表达在再灌注30分钟后开始增加，24小时达到峰值。Pearson相关分析显示，STAT 3活化与DWI异常信号面积呈负相关，与相对表观扩散系数呈正相关。这些结果表明，DWI是一个可靠的代表性梗死面积，并反映了STAT磷酸化在大鼠脑局灶性脑缺血/再灌注。
Signal transducer and activator of transcription (STAT) is a unique protein family that binds to DNA, coupled with tyrosine phosphorylation signaling pathways, acting as a transcriptional regulator to mediate a variety of biological effects. Cerebral ischemia and reperfusion can activate STATs signaling pathway, but no studies have confirmed whether STAT activation can be verified by diffusion-weighted magnetic resonance imaging (DWI) in rats after cerebral ischemia/reperfusion. Here, we established a rat model of focal cerebral ischemia injury using the modified Longa method. DWI revealed hyperintensity in parts of the left hemisphere before reperfusion and a low apparent diffusion coefficient. STAT3 protein expression showed no significant change after reperfusion, but phosphorylated STAT3 expression began to increase after 30 minutes of reperfusion and peaked at 24 hours. Pearson correlation analysis showed that STAT3 activation was correlated positively with the relative apparent diffusion coefficient and negatively with the DWI abnormal signal area. These results indicate that DWI is a reliable representation of the infarct area and reflects STAT phosphorylation in rat brain following focal cerebral ischemia/reperfusion.
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