Glucosinolate Degradation Products, Isothiocyanates, Nitriles, and Thiocyanates, Induce Stomatal Closure Accompanied by Peroxidase-Mediated Reactive Oxygen Species Production in Arabidopsis thaliana
Glucosinolate Degradation Products, Isothiocyanates, Nitriles, and Thiocyanates, Induce Stomatal Closure Accompanied by Peroxidase-Mediated Reactive Oxygen Species Production in Arabidopsis thaliana
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DOI：
10.1271/bbb.120928
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发表时间：
2013-05-01
影响因子：
1.6
通讯作者：
Murata, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Hossain, Mohammad Shakhawat;Ye, Wenxiu;Murata, Yoshiyuki
异硫氰酸盐、腈和硫氰酸盐是十字花科植物中芥子油苷的降解产物。在这项研究中，我们研究了气孔反应盟友！异硫氰酸酯（AITC）、3-丁烯腈（3BN）和硫氰酸乙酯（ESCN）。AITC，3BN，和ESCN诱导气孔关闭的野生型和atrbohD atrbohF突变体。过氧化氢酶和水杨羟肟酸（SHAM）抑制气孔关闭。降解产物诱导莲座叶细胞外活性氧（ROS）产生，诱导保卫细胞内ROS积累、NO产生和胞浆游离钙浓度（[Ca 2 +]（cyt））振荡，SHAM抑制这些变化。这些结果表明，芥子油苷降解产物诱导气孔关闭伴随着细胞外活性氧的产生SHAM敏感的过氧化物酶，细胞内活性氧积累，和[Ca 2 +]（细胞色素）振荡在拟南芥。
Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to ally! isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca2+](cyt)) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca2+](cyt) oscillation in Arabidopsis.