Cytochalasin releases mRNA from the cytoskeletal framework and inhibits protein synthesis.
Cytochalasin releases mRNA from the cytoskeletal framework and inhibits protein synthesis.
复制标题
细胞松弛素从细胞骨架框架释放 mRNA 并抑制蛋白质合成。
DOI：
10.1128/mcb.6.5.1650-1662.1986
复制
发表时间：
1986
影响因子：
5.3
通讯作者：
Penman,S
中科院分区：
文献类型：
--
作者：
Ornelles,DA;Fey,EG;Penman,S
Cytochalasin D 被证明是 HeLa 细胞中蛋白质合成的可逆抑制剂。这种抑制作用在通常用于扰乱细胞结构的药物水平上是可检测到的，并且以剂量依赖性方式增加。该药物还从细胞骨架框架释放 mRNA，与蛋白质合成的抑制成正比。体外测量的释放的 mRNA 的可翻译性没有改变，但在细胞松弛素处理的 HeLa 细胞中不再翻译。残留蛋白质合成发生在多聚核糖体上，其数量减少，但表现出正常的沉降分布。结果支持这样的假设：mRNA 与细胞骨架框架的结合是必要的，但对于翻译来说还不够。对结合多核糖体的细胞骨架框架的分析表明，用细胞松弛素 D 处理后，蛋白质的组成或数量没有变化。电子显微镜下的无包埋切片显示了该框架的详细信息。显微照片揭示了药物所影响的深刻重组，但没有表明细胞骨架元素发生实质性解聚。
Cytochalasin D was shown to be a reversible inhibitor of protein synthesis in HeLa cells. The inhibition was detectable at drug levels typically used to perturb cell structure and increased in a dose-dependent manner. The drug also released mRNA from the cytoskeletal framework in direct proportion to the inhibition of protein synthesis. The released mRNA was unaltered in its translatability as measured in vitro but was no longer translated in the cytochalasin-treated HeLa cells. The residual protein synthesis occurred on polyribosomes that were reduced in amount but displayed a normal sedimentation distribution. The results support the hypothesis that mRNA binding to the cytoskeletal framework is necessary although not sufficient for translation. Analysis of the cytoskeletal framework, which binds the polyribosomes, revealed no alterations in composition or amount of protein as a result of treatment with cytochalasin D. Electron microscopy with embedment-free sections shows the framework in great detail. The micrographs revealed the profound reorganization effected by the drug but did not indicate substantial disaggregation of the cytoskeletal elements.