Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes.
Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes.
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DOI：
10.4103/2045-9912.235128
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发表时间：
2018-04
影响因子：
2.9
通讯作者：
Miwa N
中科院分区：
文献类型：
--
作者：
Li Q;Tanaka Y;Miwa N
许多传统的分子氢研究没有研究细胞的迁移能力以及细胞凋亡与细胞骨架的关系。本文研究了含氢封闭二氧化硅微粒(H_2-SiO_2)对人正常食道上皮细胞(HEEPCs)迁移、运动和细胞骨架(F-肌动蛋白)变化的影响。结果表明，100ppm(低浓度)划痕组细胞迁移加快，微绒毛形成活跃。在进行伤口愈合试验后，10ppm和100ppm组的细胞在48小时和72小时后都表现出迁移，这表明创伤修复效果可能归因于H2-SiO_2的抗氧化能力。在划痕组，激活的caspase-3相对高水平表达，并且在300ppm以上组有增加Bax/Bcl2比率的趋势。上述结果表明，H_2-SiO_2可诱导HEEPCs，尤其是划痕细胞发生凋亡。毒性可能会导致细胞过度凋亡。此外，由于100、300和600ppm组的束蛋白/微管蛋白的比率在划痕和非划痕对照组中都有增加的趋势，因此H_2-SiO_2被认为能够促进正常细胞的束蛋白作用，并可能具有增殖作用。
Many conventional studies on molecular hydrogen have not examined cell migration ability and the relationship between apoptosis and the cytoskeleton. Here we investigated the influence of hydrogen-occluding silica microparticles (H2-silica) on cell migration motility and changes of the cytoskeleton (F-actin) in normal human esophageal epithelial cells (HEEpiCs). As the results, cell migration was promoted, and formation of microvilli was activated in the 100 ppm (low concentration) scratched group. After performing a wound healing assay, cells exhibited migration after 48 hours and 72 hours for both 10 ppm and 100 ppm groups, suggesting that the wound-repairing effects could be attributed to the antioxidant ability of H2-silica. In scratched groups, high levels of activated caspase-3 were relatively expressed and presented a tendency to increase the observed Bax/Bcl-2 ratio at more than 300 ppm groups. The above-mentioned results show that H2-silica induced apoptosis in HEEpiCs, especially in the scratched cells. Toxicity may cause an exaggerated apoptosis. Furthermore, since the ratio of fascin/tubulin in the 100, 300, and 600 ppm groups tended to increase in both the scratched and the non-scratched control groups, H2-silica was thought to be able to promote fascin action on normal cells and may be have a proliferative effect.