Generation of osteoclasts in vitro, and assay of osteoclast activity.
Generation of osteoclasts in vitro, and assay of osteoclast activity.
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DOI：
10.1007/978-1-59745-401-8_18
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发表时间：
2007
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影响因子：
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通讯作者：
N. Takahashi;N. Udagawa;Yasuhiro Kobayashi;T. Suda
中科院分区：
文献类型：
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作者：
N. Takahashi;N. Udagawa;Yasuhiro Kobayashi;T. Suda
破骨细胞是来源于单核巨噬细胞谱系的骨吸收多核细胞。作者建立了小鼠骨髓培养系统和小鼠成骨细胞与造血细胞共培养系统，其中破骨细胞是对各种成骨因子如1α，25-二羟维生素D3、甲状旁腺激素、前列腺素E2和白细胞介素-11应答的。最近的研究表明，成骨细胞表达两种破骨细胞生成所必需的细胞因子：核因子κB配体受体激活因子（RANKL）和巨噬细胞集落刺激因子（M-CSF）。使用RANKL和M-CSF，我们可以从单核巨噬细胞系细胞诱导破骨细胞，即使在没有成骨细胞的情况下。本章描述了在体外有和没有成骨细胞的情况下破骨细胞形成的方法，以及使用牙本质切片和体外形成的破骨细胞进行窝形成测定的方法。这些培养系统使得有可能分别研究破骨细胞发育和功能的每个步骤。
Osteoclasts are bone-resorbing multinucleated cells derived from the monocytemacrophage lineage. The authors have developed a mouse marrow culture system and a coculture system of mouse osteoblasts and hemopoietic cells, in which osteoclasts are formed in response to various osteotropic factors such as 1α,25-dihydroxyvitamin D3, parathyroid hormone, prostaglandin E2, and interleukin -11. Recent studies have revealed that osteoblasts express two cytokines essential for osteoclastogenesis: receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, we can induce osteoclasts from monocytemacrophage lineage cells even in the absence of osteoblasts. This chapter describes the methods for osteoclast formation in vitro in the presence and absence of osteoblasts, and for pit-formation assay using dentine slices and osteoclasts formed in vitro. These culture systems have made it possible to investigate each step of osteoclast development and function separately.