Evidence for intrinsic differences in the formation of chromatin domains in Drosophila melanogaster.
Evidence for intrinsic differences in the formation of chromatin domains in Drosophila melanogaster.
复制标题
果蝇染色质结构域形成的内在差异的证据。
DOI：
10.1093/genetics/132.4.1063
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Bishop,CP
中科院分区：
文献类型：
--
作者：
Bishop,CP
提出了对两种不同异色域形成的内在差异的研究结果。该研究利用果蝇中两种不同的位置效应杂色突变体来研究将不同 DNA 片段压缩成异染色质的过程。每一段 DNA 都编码一个影响鬃毛形态不同方面的基因。当每个基因被压缩成异染色质时，其表达就会被阻止，因此这些基因可以作为有效的报告系统来监测异染色质的扩散。两种杂色突变体在同一细胞中进行评分，从而明确消除环境和遗传背景差异。因此，在两个基因的抑制中观察到的任何差异必定是两个 DNA 片段的异染色质压缩过程中内在差异的结果。对不同杂色增强子对两个基因压缩的影响的研究表明，每个压缩事件独立于另一个发生，并且两个过程涉及不同的成分。这些结果讨论了异染色质的传播以及该过程在调节基因表达中可能发挥的作用。
The results of an investigation into intrinsic differences in the formation of two different heterochromatic domains are presented. The study utilized two different position effect variegation mutants in Drosophila melanogaster for investigating the process of compacting different stretches of DNA into heterochromatin. Each stretch of DNA encodes for a gene that affects different aspects of bristle morphology. The expression of each gene is prevented when it is compacted into heterochromatin thus the genes serve as effective reporter systems to monitor the spread of heterochromatin. Both variegating mutants are scored in the same cell such that environmental and genetic background differences are unambiguously eliminated. Any differences observed in the repression of the two genes must therefore be the result of intrinsic differences in the heterochromatic compaction process for the two stretches of DNA. Studies of the effects different enhancers of variegation have upon the compaction of the two genes indicate each compaction event occurs independently of the other, and that different components are involved in the two processes. These results are discussed with regard to spreading heterochromatin and the role this process may play in regulating gene expression.