REPELLENT OF ROOT-KNOT NEMATODES FROM EXUDATE OF HOST ROOTS
REPELLENT OF ROOT-KNOT NEMATODES FROM EXUDATE OF HOST ROOTS
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DOI：
10.1007/bf01020375
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发表时间：
1989-10-01
影响因子：
2.3
通讯作者：
DUSENBERY, DB
中科院分区：
文献类型：
--
作者：
DIEZ, JA;DUSENBERY, DB
一种趋化性试验被开发用来测量根结线虫感染幼虫对寄主根系分泌物的吸引力或排斥力。该方法具有足够的灵敏度，可用于测定25- 0.5 μ的抗虫活性。l样品的7 mM NaCl。在对不同植物根系分泌物进行不同采集方法的试验中，通常发现有回避反应，而没有吸引反应。用各种有机溶剂对番茄分泌物进行萃取，发现其驱避活性极好。在Sephadex G-15层析上，该渗出液分离成两个清晰的峰，表观分子量约为500和1000道尔顿。跑得快的峰（较大的表观尺寸）比另一个峰包含更多的活动。随后通过高效液相色谱（HPLC）在C18柱上对大峰的物质进行分析，发现其驱避活性为单峰。化学分馏均未发现可复制的引诱剂活性。
A chemotaxis assay was developed to measure attraction or repulsion of infective juveniles of the root-knot nematode Meloidogyne incognita to exudates of host roots. The assay was sufficiently sensitive to measure the repellent activity of a 25-.mu.l sample of 7 mM NaCl. In tests of root exudate collected in a variety of ways from a variety of plants, avoidance responses were usually found but attraction was not. Extraction of the exudate from tomato with various organic solvents revealed that the repellent activity was highly polar. On Sephadex G-15 chromatography this exudate separated into two clearly defined peaks with apparent molecular weights of about 500 and 1000 daltons. The faster running peak (larger apparent size) contained much more activity than the other peak. Subsequent analysis of material from the larger peak via HPLC on a C18 column revealed a single peak of repellent activity. None of the chemical fractionations uncovered reproducible attractant activity.