HELICOBACTER BILIS SP-NOV, A NOVEL HELICOBACTER SPECIES ISOLATED FROM BILE, LIVERS, AND INTESTINES OF AGED, INBRED MICE
HELICOBACTER BILIS SP-NOV, A NOVEL HELICOBACTER SPECIES ISOLATED FROM BILE, LIVERS, AND INTESTINES OF AGED, INBRED MICE
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DOI：
10.1128/jcm.33.2.445-454.1995
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发表时间：
1995-02-01
影响因子：
9.4
通讯作者：
MENDES, EN
中科院分区：
文献类型：
--
作者：
FOX, JG;YAN, LL;MENDES, EN
从老年近交系小鼠的肝脏、胆汁和下肠中分离到一种梭形细菌，其细胞周围包裹着3-14个双极鞘鞭毛和周质纤维。该菌在37℃和42℃的微好氧条件下生长，快速水解尿素，过氧化氢酶和氧化酶阳性，硝酸盐还原为亚硝酸盐，不水解乙酸吲哚和马尿酸，对头孢菌素和奈立克酸均耐药，但对甲硝唑敏感。在16S rRNA基因序列分析的基础上，将该菌归类为一种新的螺旋杆菌--胆汁螺杆菌。这种新的螺旋杆菌与肝螺杆菌一样，在小鼠的胆汁、肝脏和肠道中定居。尽管这种微生物与多灶性慢性肝炎有关，但还需要进一步的研究来确定H.Bill是否导致小鼠的慢性肝炎和/或肝细胞肿瘤。
A fusiform bacterium with 3 to 14 multiple bipolar sheathed flagella and periplasmic fibers wrapped around the cell was isolated from the liver, bile, and lower intestine of aged, inbred mice. The bacteria grew at 37 and 42 degrees C under microaerophilic conditions, rapidly hydrolyzed urea, were catalase and oxidase positive, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and were resistant to both cephalothin and nalidixic acid but sensitive to metronidazole. On the basis of 16S rRNA gene sequence analysis, the organism was classified as a novel helicobacter, Helicobacter bilis. This new helicobacter, like Helicobacter hepaticus, colonizes the bile, liver, and intestine of mice. Although the organism is associated with multifocal chronic hepatitis, further studies are required to ascertain whether H. bills is responsible for causing chronic hepatitis and/or hepatocellular tumors in mice.