Potentiation of an anthrax DNA vaccine with electroporation
Potentiation of an anthrax DNA vaccine with electroporation
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DOI：
10.1016/j.vaccine.2008.03.064
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发表时间：
2008-09-19
期刊：
影响因子：
5.5
通讯作者：
Bernard, R.
中科院分区：
文献类型：
--
作者：
Luxembourg, A.;Hannaman, D.;Bernard, R.
DNA疫苗是一种很有前途的针对生物威胁和新出现感染的免疫方法，因为它们相对容易设计、制造、储存和分发。然而，使用常规递送方法的DNA疫苗的免疫通常不能诱导一致的、稳健的免疫应答，特别是在大于小鼠的物种中。肌内（i.m.）使用电穿孔（EP）（一种有效的DNA递送方法）递送编码炭疽毒素保护性抗原（PA）的质粒，在多个实验物种中在单次免疫后2周内快速诱导抗PA IgG和毒素中和抗体。递送程序是特别剂量有效的，因此有利于在人类中疫苗施用后实现目标水平的应答。这些结果表明，EP可能是一个有价值的平台技术，用于提供针对炭疽和其他生物威胁因子的DNA疫苗。(C)2008爱思唯尔有限公司保留所有权利。
DNA vaccines are a promising method of immunization against biothreats and emerging infections because they are relatively easy to design, manufacture, store and distribute. However, immunization with DNA vaccines using conventional delivery methods often fails to induce consistent, robust immune responses, especially in species larger than the mouse. Intramuscular (i.m.) delivery of a plasmid encoding anthrax toxin protective antigen (PA) using electroporation (EP), a potent DNA delivery method, rapidly induced anti-PA IgG and toxin neutralizing antibodies within 2 weeks following a single immunization in multiple experimental species. The delivery procedure is particularly dose efficient and thus favorable for achieving target levels of response following vaccine administration in humans. These results Suggest that EP may be a valuable platform technology for the delivery of DNA vaccines against anthrax and other biothreat agents. (C) 2008 Elsevier Ltd. All rights reserved.