Pure Polyphenols and Cranberry Juice High in Anthocyanins Increase Antioxidant Capacity in Animal Organs
Pure Polyphenols and Cranberry Juice High in Anthocyanins Increase Antioxidant Capacity in Animal Organs
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DOI：
10.3390/foods8080340
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发表时间：
2019-08-01
期刊：
影响因子：
5.2
通讯作者：
Vinson, Joe
中科院分区：
文献类型：
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作者：
Bariexca, Tracey;Ezdebski, Janice;Vinson, Joe
花青素和更广泛的多酚类物质在体外是强抗氧化剂。多酚类物质是植物性食品及其饮料中的主要抗氧化剂之一。文献中有大量证据表明多酚对健康有益。为了在体内具有生物活性，它们需要是生物可利用的，并从循环运输到靶器官。迄今为止，很少有研究测试多酚，特别是花青素对动物器官抗氧化能力的影响程度。在我们的第一个试点研究中，我们研究了三种纯多酚（黄酮类化合物槲皮素，儿茶素和橙皮素）通过腹腔注射（49至63毫克/公斤）给大鼠影响其器官的抗氧化能力。随后的一项研究将一毫升100%的蔓越莓汁（花青素含量高）注射到仓鼠身上。动物器官的抗氧化能力通过使用铁还原抗氧化能力（FRAP）比色测定法对选择的大鼠器官（即，肝、肾、心脏、前列腺和脑）和仓鼠器官（即，肝、肾、心脏、膀胱和脑）。总的来说，结果显示，与对照器官相比，实验器官的抗氧化能力显著增加（p < 0.05）。通过高效液相色谱法（HPLC）对两项动物研究的器官进行分析，提供了器官提取物中多酚代谢物的证据。总之，这项研究提供的数据表明，花青素和其他多酚的管理导致两种动物模型中器官抗氧化能力的增加。这一结果支持了越来越多的证据表明，膳食多酚降低了疾病部位各种慢性疾病的风险和程度。
Anthocyanins and the broader class of polyphenols are strong antioxidants in vitro. Polyphenols are one of the major antioxidants in plant foods, and the beverages derived from them. There is extensive evidence in the literature that polyphenols are beneficial to health. In order to be bioactive in vivo, they need to be bioavailable and be transported from the circulation to target organs. To date, there have been few studies testing the extent to which polyphenols and especially anthocyanins affect the antioxidant capacity of animal organs. In our first pilot study, we investigated how three pure polyphenols (the flavonoids quercetin, catechin and hesperetin) given to rats by intraperitoneal injection (49 to 63 mg/kg) affected their organ antioxidant capacity. This was followed by a subsequent study that injected one ml of 100% cranberry juice (high in anthocyanins) to hamsters. Antioxidant capacity of animal organs was determined by using the ferric reducing antioxidant power (FRAP) colorimetric assay on methanolic extracts of select rat organs (i.e., liver, kidney, heart, prostate and brain) and in the hamster organs (i.e., liver, kidney, heart, bladder and brain). Overall the results showed that antioxidant capacity was significantly increased (p < 0.05) in experimental vs. control organs. Analysis of organs by high performance liquid chromatography (HPLC) from both animal studies provided evidence of polyphenol metabolites in the organ extracts. Taken together, this study provides data that the administration of anthocyanins and other polyphenols cause an increase in organ antioxidant capacity in two animal models. This result supports the growing evidence for the hypothesis that dietary polyphenols reduce the risk and extent of various chronic disease at the disease site.