Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression.
Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression.
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富勒烯通过调节 MAPK 通路和心脏蛋白表达介导脂肪干细胞的增殖和心肌分化
DOI：
10.2147/ijn.s95863
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发表时间：
2016
影响因子：
8
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Hao T;Zhou J;Lü S;Yang B;Wang Y;Fang W;Jiang X;Lin Q;Li J;Wang C
零维富勒烯可以调节多种细胞系的生物学行为。然而，棕色脂肪干细胞(BADSCs)的增殖和向心肌分化的作用及其分子机制尚不清楚。在本研究中，我们报道了不同浓度的富勒烯-C60对BADSCs的初步生物学效应。结果表明，富勒烯-C60在100μg/mL浓度下对BADSCs无细胞毒作用。富勒烯-C60可提高MAPK的表达水平以及干细胞的存活、增殖和心肌生成。此外，我们发现富勒烯-C60调节心肌细胞分化。富勒烯-C60促进心肌细胞特异性蛋白(cTnT和α-肌动蛋白)的表达。在较高浓度下，富勒烯-C60可随时间降低BADSCs自发心脏分化的发生率。在遗传水平上，富勒烯-C60(5μg/mL)也能促进cTnT的表达。此外，富勒烯-C60还促进细胞间缝隙连接的形成。这些发现对富勒烯在心肌梗死治疗中的临床应用具有重要意义。
Zero-dimensional fullerenes can modulate the biological behavior of a variety of cell lines. However, the effects and molecular mechanisms of proliferation and cardiomyogenic differentiation in brown adipose-derived stem cells (BADSCs) are still unclear. In this study, we report the initial biological effects of fullerene-C60 on BADSCs at different concentrations. Results suggest that fullerene-C60 has no cytotoxic effects on BADSCs even at a concentration of 100 μg/mL. Fullerene-C60 improves the MAPK expression level and stem cell survival, proliferation, and cardiomyogenesis. Further, we found that the fullerene-C60 modulates cardiomyogenic differentiation. Fullerene-C60 improves the expression of cardiomyocyte-specific proteins (cTnT and α-sarcomeric actinin). At elevated concentration, fullerene-C60 reduces the incidence of diminished spontaneous cardiac differentiation of BADSCs with time. At the genetic level, fullerene-C60 (5 μg/mL) also improves the expression of cTnT. In addition, fullerene-C60 promotes the formation of gap junction among cells. These findings have important implications for clinical application of fullerenes in the treatment of myocardial infarction.