A dansyl fluorescence-based assay for monitoring kinetics of lipid extraction and transfer.
A dansyl fluorescence-based assay for monitoring kinetics of lipid extraction and transfer.
复制标题
一种基于丹磺酰荧光的测定，用于监测脂质提取和转移的动力学。
DOI：
10.1016/j.ab.2008.07.022
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发表时间：
2008
影响因子：
2.9
通讯作者：
Fanucci,GailE
中科院分区：
文献类型：
--
作者：
Ran,Yong;Fanucci,GailE
脂质转移蛋白在细胞生物学中起着重要的作用，荧光光谱作为一种简便的时间分辨监测蛋白质-脂质相互作用的方法已经被广泛使用。在这里，我们展示了如何荧光发射特性的丹酰二氢聚乙烯可以利用表征脂质提取和脂质转移动力学。GM2激活蛋白作为脂质LTP转移蛋白的一个例子，其中独立表征从供体囊泡中提取脂质、在溶液中形成蛋白质：脂质复合物以及从复合物中释放脂质到受体脂质体的能力对于脂质转移的完整动力学表征至关重要。
Lipid transfer proteins have important roles in cellular biology, and fluorescence spectroscopy has found wide+range use as a facile means for time-resolved monitoring of protein–lipid interactions. Here, we show how the fluorescence emission properties of dansyl-DHPE can be exploited to characterize lipid extraction and lipid transfer kinetics. The GM2 activator protein serves as an example of a lipid LTP transfer protein where the ability to independently characterize lipid extraction from donor vesicles, formation of a protein:lipid complex in solution, and release of lipid from the complex to acceptor liposomes is crucial for full kinetic characterization of lipid transfer.