Chromosome pairing and meiotic recombination in Neurospora crassa spo11 mutants
Chromosome pairing and meiotic recombination in Neurospora crassa spo11 mutants
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DOI：
10.1007/s00294-006-0066-1
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发表时间：
2006-08-01
期刊：
影响因子：
2.5
通讯作者：
Catcheside, David E. A.
中科院分区：
文献类型：
--
作者：
Bowring, Frederick J.;Yeadon, P. Jane;Catcheside, David E. A.
一些生物，如哺乳动物、绿色植物和真菌，需要DNA双链断裂(DSB)才能在厚线虫的同源染色体上突触。黑腹果蝇和秀丽线虫是例外，它们独立于DSB实现突触。SPO11负责产生DSB，并可能在所有生物体中启动重组。虽然以前有人认为脉孢子虫可能不需要DSB来进行联会，但我们在这里报告了脉孢子菌sp11的突变扰乱了减数分裂，在厚壁期取消了同源染色体的联会，并导致子囊孢子经常是非整倍体的，很少存活。将sp11子囊暴露于电离辐射后，部分恢复了同系物的比对。正如预期的那样，sp11突变体的交换在脉孢子虫基因组的两个区域减少，但在第三个区域不受影响。
Some organisms, such as mammals, green plants and fungi, require double-strand breaks in DNA (DSBs) for synapsis of homologous chromosomes at pachynema. Drosophila melanogaster and Caenorhabditis elegans are exceptions, achieving synapsis independently of DSB. SPO11 is responsible for generating DSBs and perhaps for the initiation of recombination in all organisms. Although it was previously suggested that Neurospora may not require DSBs for synapsis, we report here that mutation of Neurospora spo11 disrupts meiosis, abolishing synapsis of homologous chromosomes during pachynema and resulting in ascospores that are frequently aneuploid and rarely viable. Alignment of homologues is partially restored after exposure of spo11 perithecia to ionising radiation. Crossing over in a spo11 mutant is reduced in two regions of the Neurospora genome as expected, but is unaffected in a third.